Cubillejo Isabella, Theis Kevin R, Panzer Jonathan, Luo Xixia, Banerjee Shreya, Thummel Ryan, Withey Jeffrey H
Department of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI 48201, USA.
Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Biomedicines. 2025 Jan 17;13(1):226. doi: 10.3390/biomedicines13010226.
Cholera is a diarrheal disease prevalent in populations without access to clean water. Cholera is caused by which colonizes the upper small intestine in humans once ingested. A growing number of studies suggest that the gut microbiome composition modulates animal behavior. Zebrafish are an established cholera model that can maintain a complex, mature gut microbiome during infection. Larval zebrafish, which have immature gut microbiomes, provide the advantage of high-throughput analyses for established behavioral models. We identified the effects of O1 El Tor C6706 colonization at 5 days post-fertilization (dpf) on larval zebrafish behavior by tracking startle responses at 10 dpf. We also characterized the larval gut microbiome using 16S rRNA sequencing. -infected or uninfected control groups were exposed to either an alternating light/dark stimuli or a single-tap stimulus, and average distance and velocity were tracked. : While there was no significant difference in the light/dark trial, we report a significant decrease in distance moved for C6706-colonized larvae during the single-tap trial. This suggests that early colonization of the larval gut microbiome has a dampening effect on sensorimotor function, supporting the idea of a link between the gut microbiome and behavior.
霍乱是一种在无法获得清洁水源的人群中普遍存在的腹泻疾病。霍乱由 （此处原文缺失相关病原体信息） 引起，一旦被摄入，它就会在人类的上小肠中定殖。越来越多的研究表明，肠道微生物群组成会调节动物行为。斑马鱼是一种成熟的霍乱模型，在感染期间能够维持复杂、成熟的肠道微生物群。幼体斑马鱼具有未成熟的肠道微生物群，这为已建立的行为模型提供了高通量分析的优势。我们通过追踪受精后10天（dpf）的惊吓反应，确定了受精后5天（dpf）O1 El Tor C6706定殖对幼体斑马鱼行为的影响。我们还使用16S rRNA测序对幼体肠道微生物群进行了表征。感染或未感染的对照组暴露于交替的光/暗刺激或单次轻击刺激下，并追踪平均距离和速度。 ：虽然在光/暗试验中没有显著差异，但我们报告在单次轻击试验中，定殖C6706的幼虫移动的距离显著减少。这表明幼体肠道微生物群的早期定殖对感觉运动功能有抑制作用，支持了肠道微生物群与行为之间存在联系的观点。
