Department of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, Michigan, USA.
Infect Immun. 2021 Aug 16;89(9):e0015721. doi: 10.1128/IAI.00157-21.
Zebrafish (Danio rerio) is an attractive model organism to use for an array of scientific studies, including host-microbe interactions. Zebrafish contain a core (i.e., consistently detected) intestinal microbiome consisting primarily of . Furthermore, this core intestinal microbiome is plastic and can be significantly altered due to external factors. Zebrafish are particularly useful for the study of aquatic microbes that can colonize vertebrate hosts, including Vibrio cholerae. As an intestinal pathogen, V. cholerae must colonize the intestine of an exposed host for pathogenicity to occur. Members of the resident intestinal microbial community likely must be reduced or eliminated by V. cholerae for colonization, and subsequent disease, to occur. Many studies have explored a variety of aspects of the pathogenic effects of V. cholerae on zebrafish and other model organisms but few have researched how a V. cholerae infection changes the resident intestinal microbiome. In this study, 16S rRNA gene sequencing was used to examine how five genetically diverse V. cholerae strains alter the intestinal microbiome following an infection. We found that V. cholerae colonization induced significant changes in the zebrafish intestinal microbiome. Notably, changes in the microbial profile were significantly different from each other, based on the particular strain of V. cholerae used to infect zebrafish hosts. We conclude that V. cholerae significantly modulates the zebrafish intestinal microbiota to enable colonization and that specific microbes that are targeted depend on the V. cholerae genotype.
斑马鱼（Danio rerio）是一种用于各种科学研究的有吸引力的模式生物，包括宿主-微生物相互作用。斑马鱼含有一个核心（即始终检测到）肠道微生物组，主要由 组成。此外，这个核心肠道微生物组是可塑的，可以由于外部因素而发生显著改变。斑马鱼特别适用于研究可以定殖脊椎动物宿主的水生微生物，包括霍乱弧菌。作为一种肠道病原体，霍乱弧菌必须定殖暴露宿主的肠道才能发生致病性。为了发生定植和随后的疾病，居住在肠道中的微生物群落的成员可能必须被霍乱弧菌减少或消除。许多研究已经探索了霍乱弧菌对斑马鱼和其他模式生物的致病作用的各种方面，但很少有研究研究霍乱弧菌感染如何改变居住在肠道中的微生物组。在这项研究中，使用 16S rRNA 基因测序来检查五种遗传上不同的霍乱弧菌菌株在感染后如何改变肠道微生物组。我们发现霍乱弧菌的定植诱导了斑马鱼肠道微生物组的显著变化。值得注意的是，基于用于感染斑马鱼宿主的霍乱弧菌特定菌株，微生物谱的变化彼此明显不同。我们得出的结论是，霍乱弧菌显著调节斑马鱼肠道微生物群以实现定植，并且特定的被靶向的微生物取决于霍乱弧菌的基因型。
