Yuan Ganjun, Lian Fengxian, Yan Yu, Wang Yu, Zhang Li, Zhu Jianping, Fatima Aiman, Qian Yuxing
Biotechnological Engineering Center for Pharmaceutical Research and Development, Jiangxi Agricultural University, Nanchang 330045, China.
Laboratory of Natural Medicine and Microbiological Drug, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.
Antibiotics (Basel). 2024 Dec 25;14(1):8. doi: 10.3390/antibiotics14010008.
Antimicrobial resistance (AMR) poses a significant threat to human health, and combination therapy has proven effective in combating it. It has been reported that some plant flavonoids can enhance the antibacterial effects of antibiotics and even reverse AMR. This study systematically evaluated the synergistic effects of plant flavonoids and clinical antibiotics. : The antibacterial activities of thirty-seven plant flavonoids and nine clinical antibiotics with various antimicrobial mechanisms were determined using the microbroth dilution method. Subsequently, the combined effects of twelve plant flavonoids presenting definite MICs against with these nine antibiotics were determined using the checkerboard test, together with those of thirty-two plant flavonoids presenting definite MICs against with these nine antibiotics. : Plant flavonoids in combination with antibiotics present extensive synergistic effects, and 25% of combinations exhibited synergy against and 50% against , particularly with antibiotics affecting cell membranes or ribosomes. : The findings align with the drug selection principle of synergistic combinations and suggest that plant flavonoids could extensively enhance antibiotic efficacy. Considering that various metabolites from microorganisms, plants, and animals on the Earth would definitely impact the evolution of AMR, together with the rules, key factors, and important principles of drug combination for preventing AMR, we further propose the "One Earth-One Health (OE-OH)" concept, emphasizing ecosystem interactions in AMR prevention.
抗菌耐药性（AMR）对人类健康构成重大威胁，联合治疗已被证明在对抗AMR方面有效。据报道，一些植物黄酮类化合物可以增强抗生素的抗菌效果，甚至逆转AMR。本研究系统评估了植物黄酮类化合物与临床抗生素的协同作用。：采用微量肉汤稀释法测定了37种植物黄酮类化合物和9种具有不同抗菌机制的临床抗生素的抗菌活性。随后，使用棋盘法测定了12种对这9种抗生素呈现明确最低抑菌浓度（MIC）的植物黄酮类化合物与这9种抗生素的联合效应，以及32种对这9种抗生素呈现明确MIC的植物黄酮类化合物与这9种抗生素的联合效应。：植物黄酮类化合物与抗生素联合呈现广泛的协同效应，25%的组合对[具体细菌1]表现出协同作用，50%的组合对[具体细菌2]表现出协同作用，特别是与影响细胞膜或核糖体的抗生素联合时。：这些发现与协同组合的药物选择原则一致，表明植物黄酮类化合物可以广泛增强抗生素疗效。考虑到地球上微生物、植物和动物的各种代谢产物肯定会影响AMR的进化，以及预防AMR的药物组合规则、关键因素和重要原则，我们进一步提出“同一个地球-同一个健康（OE-OH）”概念，强调在AMR预防中的生态系统相互作用。
