Zhang X, Wang L, Mu H, Wang D, Yu Y
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, China.
Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), College of Pharmaceutical Sciences, Shandong University, Jinan, China.
Lett Appl Microbiol. 2019 Jan;68(1):38-47. doi: 10.1111/lam.13084. Epub 2018 Nov 22.
Some plants of Buddleja genus possessed antimicrobial activity. In this study, the antibacterial activities of nine compounds from Buddleja albiflora were evaluated against Listeria monocytogenes, Staphyloccocus aureus, Escherichia coli and Pseudomonas aeruginosa, either alone or in combination with Erythromycin (Ery), Gentamicin (Gen) and Ampicillin (Amp). Checkerboard assay demonstrated that Kumatakenin elicited synergistic effects with all three antibiotics, and chrysin displayed synergistic effect with Ery against L. monocytogenes. Further experiments showed that the synergy effect was sufficient to eradicate biofilms formed by L. monocytogenes. Thus, our data highlighted that the combinations of secondary metabolites from B. albiflora and specific antibiotics were useful for the treatment of pathogens, which might help prevent the spread of antibiotic resistance through improving antibiotic effectiveness. SIGNIFICANCE AND IMPACT OF THE STUDY: This study measured the antibacterial activity of the metabolites from Buddleja albiflora and the synergistic interactions with antibiotics against Listeria monocytogenes on planktonic cells as well as on biofilms. The results of this study provide valuable evidence that Kumatakenin/antibiotic combination can be considered as a promising antimicrobial agent for the treatment of pathogens, which subsequently help prevent the spread of antibiotic resistance through improving antibiotic effectiveness.
一些醉鱼草属植物具有抗菌活性。在本研究中，评估了白背枫中9种化合物对单核细胞增生李斯特菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌活性，这些化合物单独使用或与红霉素（Ery）、庆大霉素（Gen）和氨苄青霉素（Amp）联合使用。棋盘法试验表明，马栗树皮素与所有三种抗生素均产生协同作用，白杨素与红霉素联合对单核细胞增生李斯特菌显示出协同作用。进一步实验表明，这种协同效应足以根除单核细胞增生李斯特菌形成的生物膜。因此，我们的数据突出表明，白背枫中的次生代谢产物与特定抗生素的组合对病原体治疗有效，这可能有助于通过提高抗生素效力来防止抗生素耐药性的传播。研究的意义和影响：本研究测定了白背枫代谢产物的抗菌活性以及与抗生素对单核细胞增生李斯特菌浮游细胞和生物膜的协同相互作用。本研究结果提供了有价值的证据，表明马栗树皮素/抗生素组合可被视为一种有前景的抗菌剂用于病原体治疗，随后通过提高抗生素效力有助于防止抗生素耐药性的传播。
