Takada Kazuya, Yoshioka Yoshie, Morikawa Kazumasa, Ariyoshi Wataru, Yamasaki Ryota
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan.
Division of Oral Functional Development, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, Fukuoka, Japan.
Antibiotics (Basel). 2025 Jan 5;14(1):36. doi: 10.3390/antibiotics14010036.
: is a leading cause of infective endocarditis (IE), which causes diverse clinical symptoms and even death. Recurrence after treatment is a crucial problem in IE, possibly caused by the presence of "persister" cells, a small bacterial population that can survive antimicrobials. In this study, the residual risk for penicillin G (PCG) and gentamicin (GM), used for treating IE, to induce persisters, was investigated. : The bactericidal effects of PCG and GM on were evaluated. Furthermore, we confirmed whether the that survived following combination treatment with PCG and GM were persisters. The bactericidal effect of the combination of PCG and GM against was measured after the addition of glucose or arginine. : Following 48 h of treatment with PCG (1600 μg/mL) and GM (64 μg/mL), survived, albeit with a low bacterial count, indicating the presence of persisters. The addition of glucose or arginine to PCG and GM increased the bactericidal effect on residual persister cells and reduced the number of persister cells. Moreover, the addition of glucose at concentrations of 10 mg/mL or higher was substantially effective in achieving sterilization. : Our findings demonstrate that persisters of that survive antimicrobial treatment may make the treatment of IE challenging, and that combining antimicrobial treatment with glucose is effective for eliminating persisters of . Taken together, these findings may facilitate the development of novel therapeutic strategies against IE caused by oral streptococcal infection.
[细菌名称]是感染性心内膜炎（IE）的主要病因，可导致多种临床症状甚至死亡。治疗后复发是IE中的一个关键问题，可能是由“持留菌”细胞的存在引起的，持留菌是一小部分能够在抗菌药物作用下存活的细菌群体。在本研究中，调查了用于治疗IE的青霉素G（PCG）和庆大霉素（GM）诱导持留菌的残留风险。评估了PCG和GM对[细菌名称]的杀菌作用。此外，我们确认了在PCG和GM联合治疗后存活的[细菌名称]是否为持留菌。在添加葡萄糖或精氨酸后，测量了PCG和GM联合对[细菌名称]的杀菌作用。用PCG（1600μg/mL）和GM（64μg/mL）治疗48小时后，[细菌名称]存活下来，尽管细菌数量较少，表明存在持留菌。在PCG和GM中添加葡萄糖或精氨酸可增强对残留持留菌细胞的杀菌作用，并减少持留菌细胞数量。此外，添加浓度为10mg/mL或更高的葡萄糖在实现杀菌方面非常有效。我们的研究结果表明，抗菌治疗后存活的[细菌名称]持留菌可能使IE的治疗具有挑战性，并且将抗菌治疗与葡萄糖联合使用可有效消除[细菌名称]持留菌。综上所述，这些发现可能有助于开发针对口腔链球菌感染引起的IE的新型治疗策略。
