Farahani Ramin M
School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
IDR/Research and Education Network, WSLHD, Westmead, NSW 2145, Australia.
Biomolecules. 2025 Jan 8;15(1):87. doi: 10.3390/biom15010087.
Mitochondrial ATP synthesis is driven by harnessing the electrochemical gradient of protons (proton motive force) across the mitochondrial inner membrane via the process of chemiosmosis. While there is consensus that the proton gradient is generated by components of the electron transport chain, the mechanism by which protons are supplied to ATP synthase remains controversial. As opposed to a global coupling model whereby protons diffuse into the intermembrane space, a localised coupling model predicts that protons remain closely associated with the lipid membrane prior to interaction with ATP synthase. Herein, a revised version of the chemiosmotic theory is proposed by introducing an RNA-based proton sink which aligns the release of sequestered protons to availability of ADP and Pi thereby maximising the efficiency of oxidative phosphorylation.
线粒体ATP合成是通过化学渗透过程利用跨线粒体内膜的质子电化学梯度（质子动力）来驱动的。虽然人们普遍认为质子梯度是由电子传递链的成分产生的，但质子供应给ATP合酶的机制仍存在争议。与质子扩散到膜间隙的全局偶联模型不同，局部偶联模型预测质子在与ATP合酶相互作用之前与脂质膜紧密结合。在此，通过引入基于RNA的质子阱提出了化学渗透理论的修订版，该质子阱使隔离质子的释放与ADP和Pi的可用性相匹配，从而最大限度地提高氧化磷酸化的效率。
