Zhao Yongying, Zhao Xianlin, Xiang Zhiguo, Zhang Dan, Yang Hongshan
Wheat Research Institute, Henan Academy of Agricultural Sciences (HAAS), Zhengzhou 450002, China.
Genes (Basel). 2025 Jan 16;16(1):90. doi: 10.3390/genes16010090.
The low molecular weight glutenin subunits (LMW-GS) of wheat have great effects on food processing quality, but the resolution of LMW-GS and the scoring of their alleles by direct analysis of proteins are difficult due to the larger number of expressed subunits and high similarity of DNA sequences. It is important to identify and classify the LMW-GS genes in order to recognize the LMW-GS alleles clearly and develop the functional markers.
The LMW-GS genes registered in GenBank were searched at NCBI, and 593 genes with complete coding sequences were obtained, including 146 , 136 , and 311 . Sequence analysis and characterization of DNA and deduced amino acids were performed using the software DNAman.
The alignment and classification showed that there were at least 9 genes with 69 allelic variants at the locus, 11 genes with 64 allelic variants at the locus, and 10 genes with 96 variants at the locus, respectively. Furthermore, the specificity of some genes and their variations was analyzed.
The results were beneficial to understanding the LMW-GS genes fully and to developing the functional markers and will provide a theoretical reference for the quality improvement of wheat variety.
小麦低分子量谷蛋白亚基（LMW-GS）对食品加工品质有重要影响，但由于表达的亚基数众多且DNA序列相似度高，通过直接分析蛋白质来解析LMW-GS及其等位基因的评分较为困难。明确LMW-GS基因并进行分类，对于清晰识别LMW-GS等位基因及开发功能标记具有重要意义。
在NCBI上搜索GenBank中注册的LMW-GS基因，获得593个具有完整编码序列的基因，包括146个、136个和311个。使用DNAman软件对DNA和推导的氨基酸进行序列分析和特征描述。
比对和分类显示，位点分别有至少9个基因和69个等位变异，位点有11个基因和64个等位变异，位点有10个基因和96个变异。此外，分析了一些基因的特异性及其变异情况。
这些结果有助于全面了解LMW-GS基因，开发功能标记，并为小麦品种品质改良提供理论参考。
