Xu Xiaodi, Zheng Xiaochuan, Zhou Qunlan, Sun Cunxin, Wang Aimin, Zhu Aimin, Zhang Yuanyuan, Liu Bo
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214128, China.
Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China.
Microorganisms. 2024 Dec 24;13(1):11. doi: 10.3390/microorganisms13010011.
The most economically important trait of the is meat quality. Protein deposition is essential in muscle growth and nutritional quality formation. The effects and potential mechanisms of feed protein sources on crustaceans' muscle protein deposition have not been elucidated. This study established an all-animal protein source (AP) and an all-plant protein source group (PP), with a feeding period of 8 weeks (four replicates per group, 45 individuals per replicate). The results demonstrated that muscle protein deposition, muscle fiber diameter, and hardness were significantly higher in the PP group ( < 0.05). The transcript levels of genes involved in protein synthesis were notably upregulated, while those of protein hydrolysis and negative regulators of myogenesis notably downregulated in PP group ( < 0.05). Furthermore, protein sources shaped differential intestinal microbiota composition and microbial metabolites profiles, as evidenced by a significant decrease in g_ ( = 0.030), and a significant increase in taurochenodeoxycholic acid (TCDCA) in PP group ( = 0.027). A significant correlation was further established by Pearson correlation analysis between the g_, TCDCA, and genes involved in the -mediated protein deposition pathway ( < 0.05). In vitro anaerobic fermentation confirmed the ability of the two groups of intestinal flora to metabolically produce differential TCDCA ( = 0.038). Our results demonstrated that the '-TCDCA-' axis may mediate the effects of different protein sources on muscle development and protein deposition in , which was anticipated to represent a novel target for the muscle quality modulation in crustaceans.
[该生物]最重要的经济性状是肉质。蛋白质沉积对于肌肉生长和营养品质形成至关重要。饲料蛋白质来源对甲壳类动物肌肉蛋白质沉积的影响及其潜在机制尚未阐明。本研究设立了全动物蛋白源组（AP）和全植物蛋白源组（PP），饲养期为8周（每组4个重复，每个重复45只个体）。结果表明，PP组的肌肉蛋白质沉积、肌纤维直径和硬度显著更高（P<0.05）。PP组中参与蛋白质合成的基因转录水平显著上调，而蛋白质水解和肌肉生成负调控因子的转录水平显著下调（P<0.05）。此外，蛋白质来源塑造了不同的肠道微生物群组成和微生物代谢物谱，PP组中g_显著降低（P = 0.030），牛磺鹅去氧胆酸（TCDCA）显著增加（P = 0.027）证明了这一点。通过Pearson相关性分析进一步确定了g_、TCDCA与参与[该生物]介导的蛋白质沉积途径的基因之间存在显著相关性（P<0.05）。体外厌氧发酵证实了两组肠道菌群代谢产生不同TCDCA的能力（P = 0.038）。我们的结果表明，“[该生物]-TCDCA-”轴可能介导不同蛋白质来源对[该生物]肌肉发育和蛋白质沉积的影响，这有望成为甲壳类动物肌肉品质调控的新靶点。
