Yin Hongbin, Xu Mingyi, Huang Qingyang, Xie Lihong, Yang Fan, Zhang Chao, Sha Gang, Cao Hongjie
Institute of Natural Resources and Ecology, Heilongjiang Academy of Science, Harbin 150040, China.
National and Local Joint Laboratory of Wetland and Ecological Conservation, Harbin 150040, China.
Microorganisms. 2025 Jan 1;13(1):56. doi: 10.3390/microorganisms13010056.
Increasing nitrogen (N) addition induces soil nutrient imbalances and is recognized as a major regulator of soil microbial communities. However, how soil bacterial abundance, diversity, and community composition respond to exogenous N addition in nutrient-poor and generally N-limited regions remains understudied. In this study, we investigated the effects of short-term exogenous N additions on soil bacterial communities using quantitative polymerase chain reaction (PCR) and Illumina Miseq sequencing in an in situ N addition field experiment. The results showed that a low nitrogen addition increased the observed species (Sobs) of the bacterial community, and with the increased nitrogen addition, the Sobs of bacteria gradually decreased, especially the unique OTUs. The relative abundance of , , and increased with increasing nitrogen addition, whereas the relative abundance of and decreased. Soil properties play an important role in bacterial community structure at phylum or genus levels. Short-term nitrogen addition increased the proportion of nodes from and in the co-occurrence network and enhanced the stability of the microbial network. may play an important role in constructing the network. Our study aims to explore the effects of nitrogen addition on the diversity, composition, and structure of soil bacterial communities in nutrient-poor areas caused by ecological disturbances.
增加氮（N）添加会导致土壤养分失衡，并且被认为是土壤微生物群落的主要调节因子。然而，在养分贫瘠且普遍氮限制的地区，土壤细菌丰度、多样性和群落组成如何响应外源氮添加仍未得到充分研究。在本研究中，我们在原位氮添加田间试验中，使用定量聚合酶链反应（PCR）和Illumina Miseq测序技术，研究了短期外源氮添加对土壤细菌群落的影响。结果表明，低氮添加增加了细菌群落的观测物种数（Sobs），随着氮添加量增加，细菌的Sobs逐渐减少，尤其是独特的操作分类单元（OTUs）。随着氮添加量增加，[具体菌属名称1]、[具体菌属名称2]和[具体菌属名称3]的相对丰度增加，而[具体菌属名称4]和[具体菌属名称5]的相对丰度降低。土壤性质在门或属水平的细菌群落结构中起重要作用。短期氮添加增加了共现网络中[具体菌门名称1]和[具体菌门名称2]的节点比例，并增强了微生物网络的稳定性。[具体菌属名称6]可能在构建网络中起重要作用。我们的研究旨在探讨氮添加对生态干扰导致的养分贫瘠地区土壤细菌群落多样性、组成和结构的影响。
