Quorum Sensing Mediates Interaction with In Vitro.

and are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that inhibits the growth of by iron chelating via quorum sensing. Next, through proteomic analysis, we discovered that the PQS molecule significantly changed a large amount of protein expression in , including proteins involved in the type VII secretion system and iron homeostasis. Furthermore, we revealed that PQS significantly enhanced the production of bacterial membrane vesicles (MVs) by . Our study suggests that the PQS can serve as an interspecies signaling molecule to communicate with and affect their physiology and virulence.