Choi Jihye, Jang Yuseong, Paik Hyeon-Gi, Ha Melissa Hyun-Joo, Kwon Jungkee
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan-si 54596, Jeollabuk-do, Republic of Korea.
Broccos, Room 503, 3-3 Jongangdae-ro, 296 beon-gil, Dong-gu, Busan 48730, Republic of Korea.
Medicina (Kaunas). 2025 Jan 7;61(1):89. doi: 10.3390/medicina61010089.
: Acute gastric injury is a prevalent gastrointestinal disorder characterized by inflammation and damage to the stomach lining. In this study, we investigated the therapeutic potential effects of broccoli stem extract (BSE) against acute gastritis in a rat model. : The antioxidant properties of BSE were evaluated through DPPH and ABTS radical scavenging activity assays and total polyphenol content analysis. Acute gastric injury was induced using 150 mM HCl/60% EtOH, and male SD rats (6-weeks old, = 6/group) were administered BSE by oral gavage at concentrations of 50, 125, and 250 mg/kg. : The BSE 250 mg/kg group exhibited significant relief of clinical signs compared to the negative control group. In addition, the BSE 250 mg/kg group showed significant improvements in gastric tissue, including macroscopic reductions in ulcer size and improved overall gastric morphology as assessed through gross examination, as well as microscopic improvements such as reduced inflammation and the restoration of mucosal integrity observed in histopathological analysis. BSE modulated NF-κB signaling, decreased inflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased PGE levels. Pyloric ligation experiments demonstrated reduced pepsin and gastric acid secretion. Improvements in gastric emptying and gastrointestinal motility were also observed in the BSE-treated group. : These findings highlight the potential of BSE as an effective therapeutic agent for acute gastritis in rats, offering significant improvements in gastric damage, inflammation, and motility.
急性胃损伤是一种常见的胃肠道疾病，其特征为胃黏膜的炎症和损伤。在本研究中，我们在大鼠模型中研究了西兰花茎提取物（BSE）对急性胃炎的潜在治疗作用。通过DPPH和ABTS自由基清除活性测定以及总多酚含量分析评估了BSE的抗氧化特性。使用150 mM HCl/60%乙醇诱导急性胃损伤，对6周龄雄性SD大鼠（每组n = 6）以50、125和250 mg/kg的浓度通过口服灌胃给予BSE。与阴性对照组相比，250 mg/kg BSE组的临床症状明显缓解。此外，250 mg/kg BSE组的胃组织有显著改善，包括通过大体检查评估的溃疡大小宏观减小和整体胃形态改善，以及在组织病理学分析中观察到的微观改善，如炎症减轻和黏膜完整性恢复。BSE调节NF-κB信号通路，降低炎症细胞因子（TNF-α、IL-1β和IL-6）水平，并提高PGE水平。幽门结扎实验表明胃蛋白酶和胃酸分泌减少。在BSE治疗组中还观察到胃排空和胃肠动力的改善。这些发现突出了BSE作为大鼠急性胃炎有效治疗剂的潜力，在胃损伤、炎症和动力方面有显著改善。
