哇巴因在尼日利亚菌素存在的情况下对小鼠腹水肿瘤细胞摄取氨基酸的影响。
Effect of ouabain on amino acid uptake by mouse ascites-tumour cells in the presence of nigericin.
作者信息
Johnson E, Eddy A A
出版信息
Biochem J. 1985 Mar 15;226(3):773-9. doi: 10.1042/bj2260773.
Abstract
Mouse ascites-tumour cells oxidizing lactate, in a modified Ringer solution, concentrated 2-aminoisobutyrate, L-methionine or 2-(methylamino)isobutyrate about 20-fold from a 0.4 mM solution in the presence of 2-3 micrograms of nigericin/mg cellular dry wt. The ionophore increased cellular [Na+] to almost 100 mM when extracellular [Na+] was about 45 mM. Either valinomycin or the two mitochondrial inhibitors oligomycin and antimycin acting together each markedly lowered the extent to which the tumour cells concentrated amino acid, from the above factor of about 20 to roughly 2-fold. Ouabain (1 mM) had a similar effect, and further raised cellular [Na+]. The sodium pump appeared to be closely involved in amino acid uptake under these conditions.
摘要
在改良的林格氏溶液中，小鼠腹水肿瘤细胞氧化乳酸时，在每毫克细胞干重存在2 - 3微克尼日利亚菌素的情况下，能将0.4 mM溶液中的2 - 氨基异丁酸、L - 甲硫氨酸或2 -（甲氨基）异丁酸浓缩约20倍。当细胞外[Na⁺]约为45 mM时，离子载体使细胞内[Na⁺]增加到近100 mM。缬氨霉素或两种线粒体抑制剂寡霉素和抗霉素共同作用，均显著降低肿瘤细胞浓缩氨基酸的程度，从上述约20倍降至约2倍。哇巴因（1 mM）有类似作用，并进一步提高细胞内[Na⁺]。在这些条件下，钠泵似乎与氨基酸摄取密切相关。
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Biochim Biophys Acta. 1963 Aug 13;74:392-400. doi: 10.1016/0006-3002(63)91383-0.
2
Energization of amino acid transport in energy-depleted Ehrlich cells and plasma membrane vesicles.
Biochim Biophys Acta. 1980 Jun 20;599(1):175-90. doi: 10.1016/0005-2736(80)90066-8.
3
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J Biol Chem. 1980 Aug 10;255(15):7311-6.
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