胆囊收缩素（CCK）58氨基末端片段在大鼠脑中的色谱特征分布。

The distribution of chromatographic characterization of an amino-terminal fragment of cholecystokinin (CCK) 58 in rat brain.

作者信息

Beinfeld M C

出版信息

Biochem Biophys Res Commun. 1985 Mar 29;127(3):720-5. doi: 10.1016/s0006-291x(85)80002-4.

Abstract

Utilizing a specific antiserum against CCK 58, a single immunoreactive peptide of about 1750 daltons was detected in rat brain extracts. It is distributed in all rat brain regions containing CCK 8, though it is most abundant in areas where CCK terminals predominate (septum, striatum and olfactory tubercle/nucleus accumbens). Based on its molecular weight, it is probably the amino terminal portion of CCK 58 left when CCK 39 is cleaved, and it may represent an intermediate in the processing of pre-pro-CCK. The presence of this peptide in CCK terminal areas implies that the proteolytic cleavage of CCK 58 occurs late in the processing, possibly in synaptic vesicles. It is also possible that this peptide can be released along with CCK 8 and exert an influence on synaptic transmission.

摘要

利用针对CCK 58的特异性抗血清，在大鼠脑提取物中检测到一种约1750道尔顿的单一免疫反应性肽。它分布于所有含有CCK 8的大鼠脑区，不过在CCK终末占主导的区域（隔区、纹状体和嗅结节/伏隔核）最为丰富。基于其分子量，它可能是CCK 58在裂解产生CCK 39后剩余的氨基末端部分，并且可能代表前CCK原加工过程中的一个中间体。该肽在CCK终末区域的存在意味着CCK 58的蛋白水解裂解发生在加工后期，可能在突触小泡中。这种肽也有可能与CCK 8一起释放并对突触传递产生影响。