Gaudreau P, Quirion R, St-Pierre S, Pert C B
Peptides. 1983 Sep-Oct;4(5):755-62. doi: 10.1016/0196-9781(83)90032-3.
[3H]Pentagastrin binds specifically to an apparent single class of CCK receptors on slide-mounted sections of rat brain (KD = 5.6 nM; Bmax = 36.6 fmol/mg protein). This specific binding is temperature-dependent and regulated by ions and nucleotides. The relative potencies of C-terminal fragments of CCK-8(SO3H), benzotript and proglumide in inhibiting specific [3H]pentagastrin binding to CCK brain receptors reinforce the concept of different brain and pancreas CCK receptors. CCK receptors were visualized by using tritium-sensitive LKB film analyzed by computerized densitometry. CCK receptors are highly concentrated in the cortex, dentate gyrus, granular and external plexiform layers of the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, claustrum, accumbens nucleus, some nuclei of the amygdala, thalamus and hypothalamus.
[3H]五肽胃泌素特异性结合大鼠脑冰冻切片上一类明显单一的胆囊收缩素（CCK）受体（解离常数KD = 5.6纳摩尔；最大结合量Bmax = 36.6飞摩尔/毫克蛋白质）。这种特异性结合依赖于温度，并受离子和核苷酸调节。CCK-8（SO3H）的C末端片段、苯佐曲平及丙谷胺在抑制[3H]五肽胃泌素与CCK脑受体的特异性结合方面的相对效价，强化了脑和胰腺中不同CCK受体的概念。通过使用经计算机密度测定分析的对氚敏感的LKB胶片来观察CCK受体。CCK受体高度集中于大脑皮层、齿状回、嗅球的颗粒层和外丛状层、前嗅核、嗅结节、屏状核、伏隔核、杏仁核的一些核团、丘脑和下丘脑。
