Remnitz Alexandra D, Hadad Roey, Keane Robert W, Dietrich W Dalton, de Rivero Vaccari Juan Pablo
Department of Neurological Surgery and the Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Int J Mol Sci. 2025 Jan 20;26(2):831. doi: 10.3390/ijms26020831.
Interleukin (IL)-1β is a pro-inflammatory cytokine whose levels are increased in the brains of Alzheimer's disease (AD) patients. Despite the role of IL-1β in the pathology of AD, the fact that it is expressed at very low levels makes it a challenging cytokine to measure, hence limiting its potential use as a reliable biomarker. Moreover, being able to accurately and reliably measure the levels of IL-1 β in blood makes it possible to evaluate this cytokine as a potential biomarker of the inflammatory response in AD. In this study, we compared three quantification methodologies, Meso-Scale Discovery (MSD), both V-Plex and S-Plex versions, and Quanterix's SIMOA (Single-Molecule Array), to measure IL-1β in the serum of AD patients and age-matched controls. These assays are routinely used to measure IL-1β serum levels with high specificity and sensitivity in human AD patients, yet to the best of our knowledge, no study has compared all three techniques for their accuracy to measure IL-1β as biomarkers. Our findings indicate the two MSD assays can be used to measure IL-1β levels in AD and control serum, but the SIMOA assay showed the highest receiver operating characteristics (ROCs), with an area under the curve (AUC) of 0.9532, which can be compared to the AUC values for the V-Plex assay, 0.5660, and the S-Plex assay, 0.6632. Taken together, these data show that although all technologies are useful in the measurement of IL-1β in the blood, the SIMOA IL-1β 3.0 assay is more reliable and sensitive in measuring biomarkers of AD.
白细胞介素（IL）-1β是一种促炎细胞因子，在阿尔茨海默病（AD）患者的大脑中其水平会升高。尽管IL-1β在AD病理过程中发挥作用，但它表达水平极低这一事实使其成为一种难以测量的细胞因子，从而限制了其作为可靠生物标志物的潜在用途。此外，能够准确可靠地测量血液中IL-1β的水平使得评估这种细胞因子作为AD炎症反应的潜在生物标志物成为可能。在本研究中，我们比较了三种定量方法，即Meso-Scale Discovery（MSD）的V-Plex和S-Plex版本，以及Quanterix公司的SIMOA（单分子阵列），以测量AD患者和年龄匹配对照组血清中的IL-1β。这些检测方法通常用于在人类AD患者中高特异性和高灵敏度地测量IL-1β血清水平，但据我们所知，尚无研究比较这三种技术测量IL-1β作为生物标志物的准确性。我们的研究结果表明，两种MSD检测方法可用于测量AD和对照血清中的IL-1β水平，但SIMOA检测方法显示出最高的受试者工作特征（ROC），曲线下面积（AUC）为0.9532，与之相比，V-Plex检测方法的AUC值为0.5660，S-Plex检测方法的AUC值为0.6632。综上所述，这些数据表明，尽管所有技术在测量血液中的IL-1β方面都有用，但SIMOA IL-1β 3.0检测方法在测量AD生物标志物方面更可靠、更灵敏。
