Liu Min-Xuan, Xu Xiao-Pei, Meng Fan-Ming, Zhang Bing, Li Wei-Gang, Zhang Yuan-Yuan, Zen Qiao-Ying, Liu Wen-Ge
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China.
Life (Basel). 2025 Jan 6;15(1):59. doi: 10.3390/life15010059.
is used as an experimental animal model for the study of three-host ticks due to its special life cycle and easy maintenance in the laboratory and in its reproduction. The life cycle of goes through a tightly regulated life cycle to adapt to the changing host and environment, and these stages of transition are also accompanied by proteome changes in the body. Here, we used the isobaric tags for a relative and absolute quantification (iTRAQ) technique to systematically describe and analyze the dynamic expression of the protein and the molecular basis of the proteome of in seven differential developmental stages (eggs, unfed larvae, engorged larvae, unfed nymphs, engorged nymphs unfed adults, and engorged adults). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed proteins (DEPs) were used. In our study, A total of 2044 proteins were identified, and their expression profiles were classified at different developmental stages. In addition, it was found that tissue and organ development-related proteins and metabolism-related proteins were involved in different physiological processes throughout the life cycle through the GO and KEGG analysis of DEPs. More importantly, we found that the up-regulated proteins of engorged adult ticks were mainly related to yolk absorption, degradation, and ovarian development-related proteins. The abundance of the cuticle proteins in the unfed stages was significantly higher compared with those of the engorged ticks in the previous stages. We believe that our study has made a significant contribution to the research on , which is an important vector of SFTSV. In this study, we identified changes in the proteome throughout the development, and functional analysis highlighted the important roles of many key proteins in developmental events (ovarian development, the molting process, the development of midgut, the development and degeneration of salivary glands, etc.). The revelation of this data will provide a reference proteome for future research on tick functional proteins and candidate targets for elucidating development and developing new tick control strategies.
由于其特殊的生命周期以及在实验室中易于饲养和繁殖，被用作研究三宿主蜱的实验动物模型。蜱的生命周期经历严格调控以适应不断变化的宿主和环境，而这些转变阶段身体中也伴随着蛋白质组的变化。在此，我们使用等压标签相对和绝对定量（iTRAQ）技术，系统地描述和分析了蜱在七个不同发育阶段（卵、未进食幼虫、饱血幼虫、未进食若虫、饱血若虫、未进食成虫和饱血成虫）蛋白质的动态表达以及蛋白质组的分子基础。对差异表达蛋白（DEPs）进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。在我们的研究中，共鉴定出2044种蛋白质，并对它们在不同发育阶段的表达谱进行了分类。此外，通过对DEPs的GO和KEGG分析发现，组织和器官发育相关蛋白以及代谢相关蛋白参与了整个生命周期的不同生理过程。更重要的是，我们发现饱血成虫蜱上调的蛋白质主要与卵黄吸收、降解以及卵巢发育相关蛋白有关。与前一阶段的饱血蜱相比，未进食阶段表皮蛋白的丰度显著更高。我们相信我们的研究对蜱（一种严重发热伴血小板减少综合征病毒的重要传播媒介）的研究做出了重大贡献。在本研究中，我们鉴定了蜱整个发育过程中蛋白质组的变化，功能分析突出了许多关键蛋白在发育事件（卵巢发育、蜕皮过程、中肠发育、唾液腺发育和退化等）中的重要作用。这些数据的揭示将为未来蜱功能蛋白的研究提供参考蛋白质组，并为阐明蜱的发育和开发新的蜱控制策略提供候选靶点。
