透明质酸在酸性介质中的构象变化（作者译）

[Conformational changes of hyaluronic acid in acid medium (author's transl)].

作者信息

Hofmann H, Schmut O

出版信息

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1976 Jan 16;198(1):95-100. doi: 10.1007/BF00411451.

Abstract

Both vitreous body homogenate and a fibrous product containing hyaluronic acid and collagen isolated from vitreous humor by acetone precipitation were investigated to elucidate the liquefaction of vitreous body by acids. Graphic evaluation of the titration with hydrochlorid acid and viscosity measurements suggest a changed of the hyaluronic acid molecule in the pH-range between 5.2 and 4.1. Between these pH-values the ionization of carboxyl groups is decreased accompanied by conformational changes of the hyaluronic acid molecule. These results are in agreement with the conclusion of other authors that by lowering the pH of hyaluronic acid solutions a random coil to double helix transition of hyaluronic acid occurs.

摘要

为了阐明玻璃体在酸作用下的液化过程，对玻璃体匀浆以及通过丙酮沉淀从玻璃体液中分离出的含有透明质酸和胶原蛋白的纤维状产物进行了研究。用盐酸滴定的图形评估和粘度测量表明，在pH值5.2至4.1之间，透明质酸分子发生了变化。在这些pH值之间，羧基的电离减少，同时伴随着透明质酸分子的构象变化。这些结果与其他作者的结论一致，即通过降低透明质酸溶液的pH值，透明质酸会发生从无规卷曲到双螺旋的转变。