Ye Dongyan, Li Qianhui, Liu Xuwen, Zhou Jie, Yin Shuren, Zhang Suyi, Wang Jing, Zaman Kh Ahammad Uz, Bai Helong, Meng Fanlei
College of Chemistry, Changchun Normal University, Changchun 130032, China.
Technical Innovation Laboratory for Research and Development of Economic Plants and Edible and Medicinal Fungi in Cold Regions of Jilin Province, Changchun 130032, China.
Molecules. 2025 Jan 8;30(2):220. doi: 10.3390/molecules30020220.
The Compendium of Materia Medica highlights the therapeutic properties of (). In this study, the species and content of volatile components, inorganic elements, and amino acids were measured, and the activity of crude extracts of ethanol and water was studied. GC-MS analysis revealed 37-53 components across different life stages, excluding excessive heavy metals and containing essential trace elements. Amino acid profiling identified 15 types, rich in seven medicinal varieties. The in vitro anti-tumor testing of ethanol extracts from adult males (TDA (M)) and adult females (TDA (F)) was also performed. The experimental results showed that TDA (M) exhibited growth inhibition rates of 86.22% ± 1.19%, 85.42% ± 0.63%, 88.15% ± 3.27%, and 97.23% ± 0.60% against MKN-45, K-562, 239T, and 5637 tumor cells, respectively, while TDA (F) showed 81.28% ± 5.06% inhibition against K-562 cells. TDA best protected cells induced by HO at the concentration of 12.5 μg/mL, achieving a cell survival rate of 81%. Overall, TDA, TDA(F), and TDA(M) have notable anti-tumor and antioxidant activity, highlighting the significance of chemical analysis for their potential bioactivity.
《本草纲目》突出了（）的治疗特性。在本研究中，测定了挥发性成分、无机元素和氨基酸的种类及含量，并研究了乙醇提取物和水提取物的活性。气相色谱 - 质谱联用分析显示，不同生长阶段有37 - 53种成分，不含有过量重金属且含有必需微量元素。氨基酸谱分析鉴定出15种类型，在7个药用品种中含量丰富。还对成年雄性（TDA（M））和成年雌性（TDA（F））的乙醇提取物进行了体外抗肿瘤测试。实验结果表明，TDA（M）对MKN - 45、K - 562、239T和5637肿瘤细胞的生长抑制率分别为86.22%±1.19%、85.42%±0.63%、88.15%±3.27%和97.23%±0.60%，而TDA（F）对K - 562细胞的抑制率为81.28%±5.06%。TDA在浓度为12.5μg/mL时对过氧化氢诱导的细胞具有最佳保护作用，细胞存活率达到81%。总体而言，TDA、TDA（F）和TDA（M）具有显著的抗肿瘤和抗氧化活性，突出了化学分析对其潜在生物活性的重要性。
