Spiegel Christopher, Ünalan Burak, Kaserbacher Andreas, Arora Rohit, Coraça-Huber Débora C
Research Laboratory for Biofilms and Implant Associated Infections (BIOFILM LAB), University Hospital for Orthopaedics and Traumatology, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.
Department of Orthopaedics and Traumatology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria.
Pathogens. 2025 Jan 10;14(1):57. doi: 10.3390/pathogens14010057.
The burden of prosthetic joint infection in combination with antibiotic-resistant bacterial strains is a rising dilemma for patients experiencing total joint replacements. Around 0.8-2% of patients experience prosthetic joint infections, while up to 21% of patients are considered fatal cases after 5 years. is one of the main reasons for prosthetic joint infections. Its capability of forming biofilms and developing mechanisms against antibiotics is one of the most dangerous clinical topics being currently discussed. Previous studies have shown the promising results of omega-3 fatty acids as an antimicrobial agent against . Though an antimicrobial effect has been examined, the influence of polyunsaturated fatty acids on in the presence of human osteoblasts has not been reported yet. In this study, we aimed to investigate the influence of omega-3 fatty acids on the biofilm formation of ATCC 29213 in the presence of cells. The co-culture setup helped to examine the influence of omega-3 fatty acids on the race for surface to simulate prosthetic joint infections.
In this study, we tested ATCC 29213 co-cultured with human fetal osteoblasts in the presence of sub-MIC and MIC concentrations of docosahexaenoic acid (1.25 mg/L, 2.5 mg/L) and eicosapentaenoic acid (0.15 mg/L, 0.3 mg/L) after 1, 6 and 24 h of incubation. After establishing the co-culture, cell culture and biofilm, we performed colony-forming unit counting and cell counting to examine cell survivability. In addition, we carried out scanning electron microscopy to study the race for surface behaviour of the cells.
We found a protective influence of omega-3 fatty acids on osteoblasts when present in co-culture with after 6 h of incubation. Omega-3 fatty acids increase the cell survival of osteoblasts after 6 h in a co-culture with bacteria and are able to influence the race for surface. In this study, the strain of ATCC 29213 showed signs of growth inhibition within the first 6 h.
Omega-3 fatty acids can be a valuable antimicrobial agent in terms of decreasing the risk of on-site infection during surgery. Omega-3 fatty acids were shown to decrease the bacterial load within the first 6 h of incubation and increase the survivability of osteoblasts.
对于接受全关节置换的患者而言，人工关节感染与抗生素耐药菌株共同构成的负担是一个日益严峻的难题。约0.8%-2%的患者会发生人工关节感染，而高达21%的患者在5年后被视为致命病例。 是人工关节感染的主要原因之一。其形成生物膜以及产生抗生素耐药机制的能力是当前正在讨论的最危险的临床话题之一。先前的研究已表明ω-3脂肪酸作为一种抗 抗菌剂具有良好前景。尽管已检测到其抗菌效果，但多不饱和脂肪酸在人成骨细胞存在的情况下对 的影响尚未见报道。在本研究中，我们旨在探究ω-3脂肪酸在人成骨细胞存在的情况下对金黄色葡萄球菌ATCC 29213生物膜形成的影响。共培养设置有助于检测ω-3脂肪酸对争夺表面的影响，以模拟人工关节感染。
在本研究中，我们检测了在亚抑菌浓度和抑菌浓度的二十二碳六烯酸（1.25毫克/升、2.5毫克/升）和二十碳五烯酸（0.15毫克/升、0.3毫克/升）存在的情况下，与人类胎儿成骨细胞共培养1、6和24小时后的金黄色葡萄球菌ATCC 29213。在建立共培养、细胞培养和生物膜后，我们进行了菌落形成单位计数和细胞计数以检测细胞存活率。此外，我们进行了扫描电子显微镜检查以研究细胞争夺表面的行为。
我们发现，在孵育6小时后，当ω-3脂肪酸与金黄色葡萄球菌共培养时，对成骨细胞有保护作用。在与细菌共培养6小时后，ω-3脂肪酸可提高成骨细胞的存活率，并能够影响争夺表面的情况。在本研究中，金黄色葡萄球菌ATCC 29213菌株在前6小时内显示出生长抑制迹象。
就降低手术期间局部感染风险而言，ω-3脂肪酸可能是一种有价值的抗菌剂。研究表明，ω-3脂肪酸在孵育的前6小时内可降低细菌载量，并提高成骨细胞的存活率。
