Marcinčáková Dana, Hudáková Nikola, Miłek Michal, Kolesárová Mária, Dżugan Małgorzata, Cizkova Dasa, Legáth Jaroslav
Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Komenského 73, 041 81 Kosice, Slovakia.
Centre of Experimental and Clinical Regenerative Medicine, Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
Pharmaceuticals (Basel). 2024 Dec 28;18(1):27. doi: 10.3390/ph18010027.
The health benefits of honeybee products and herbs are well known, and their appropriate combination may enhance their biological efficacy. This study investigated the biological properties of a combined barberry root and propolis extract (PBE) in comparison to a propolis extract (PE), a barberry root extract (BE), and pure berberine (BN). The antioxidant properties were evaluated using DPPH and FRAP methods and total phenolic contents (TPC) were assessed by the Folin-Ciocalteu method. HPTLC was used to quantify the BE in the tested samples. Their effect on HEK293T cells was monitored in real-time by using the xCELLigence system which recorded changes in the proliferative activity (PA). The metabolic activity (MA) was evaluated using an MTS test and cell migration was analyzed via a scratch assay. The PE exhibited a higher TPC (198.67 mg/g) than the BE (119.3 mg/g). The PBE exhibited a comparable antioxidant effect to that of the PE. In the cell assays, the PE, the BE, and BN significantly reduced the proliferative activity at higher concentrations ( < 0.0001) while the PBE demonstrated a lower cytotoxicity and proved to be safer for the tested cells. The highest IC value was determined for the PBE (130 µg/mL), suggesting that this combination has a reduced cytotoxicity. However, the scratch test did not confirm a significant supportive effect of the PBE on cell migration. Although the PBE did not show enhanced antioxidant properties, it may mitigate cytotoxicity and support proliferation at lower concentrations. This suggests that extraction of raw propolis with a previously prepared barberry extract results in a safer preparation, but its therapeutic potential requires further studies using biological models.
蜜蜂产品和草药对健康的益处广为人知，它们的适当组合可能会增强其生物学功效。本研究调查了刺黄柏根与蜂胶提取物（PBE）组合的生物学特性，并与蜂胶提取物（PE）、刺黄柏根提取物（BE）和纯黄连素（BN）进行了比较。使用DPPH和FRAP方法评估抗氧化性能，并通过福林-西奥尔特法评估总酚含量（TPC）。采用高效薄层色谱法（HPTLC）对测试样品中的BE进行定量。使用xCELLigence系统实时监测它们对HEK293T细胞的影响，该系统记录增殖活性（PA）的变化。使用MTS试验评估代谢活性（MA），并通过划痕试验分析细胞迁移。PE的TPC（198.—— 67mg/g）高于BE（119.3mg/g）。PBE表现出与PE相当的抗氧化作用。在细胞试验中，PE、BE和BN在较高浓度下（<0.0001）显著降低增殖活性，而PBE表现出较低的细胞毒性，对测试细胞更安全。PBE的最高半数抑制浓度（IC）值为130μg/mL，表明这种组合的细胞毒性降低。然而，划痕试验未证实PBE对细胞迁移有显著的促进作用。尽管PBE没有表现出增强的抗氧化性能，但它可能在较低浓度下减轻细胞毒性并支持细胞增殖。这表明用预先制备的刺黄柏提取物提取生蜂胶会得到更安全的制剂，但其治疗潜力需要使用生物学模型进行进一步研究。
