Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil.
Brazilian Society of Plastic Surgery - SBCP, Rua Funchal 129, 2º andar, Vila Olímpia, 04551-060 São Paulo, SP, Brazil.
An Acad Bras Cienc. 2024 Aug 16;96(suppl 1):e20240100. doi: 10.1590/0001-3765202420240100. eCollection 2024.
The use of platelet-rich plasma (PRP) and adipose-derived stromal cells (ADSC) have been investigated as a form of wound healing enhancement. The objective of this work was to evaluate the association of red propolis (RP) and PRP as inducers of ADSC for application in tissue regeneration. Adipose tissue post-collection and post-cryopreservation was isolated with type II collagenase, characterized by flow cytometry, and differentiated into osteogenic, chondrogenic and adipose cell. The viability of ADSC was evaluated when exposed to different concentrations of RP using the MTT and trypan blue assay. Acridine orange and ethidium bromide (AO/EB) was performed to evaluate cell death events. Horizontal migration methods were investigated in ADSC using autologous and homologous PRP associated with RP (PRP/RP). All assays were processed in triplicate. Flow cytometry and cellular differentiation showed that type II collagenase was effective for isolating ADSC post-collection and post-cryopreservation. RP extracts at concentrations of up to 50 μg.mL-1 presented no cytotoxic effects. Association of PRP and RP at 25 and 50 μg.ml-1 influenced ADSC migration, with total closure on the seventh day after exposition. The results here presented could stimulate proliferation of ADSC cells that may contribute directly or indirectly to the reconstructive process of tissue regeneration.
富血小板血浆（PRP）和脂肪来源的基质细胞（ADSC）已被研究为一种增强伤口愈合的形式。本工作的目的是评估红蜂胶（RP）和 PRP 作为 ADSC 诱导物在组织再生中的应用的相关性。收集和冷冻保存后的脂肪组织用 II 型胶原酶分离，通过流式细胞术进行表征，并分化为成骨细胞、软骨细胞和成脂细胞。使用 MTT 和台盼蓝测定法评估暴露于不同浓度 RP 时 ADSC 的活力。吖啶橙和溴化乙锭（AO/EB）用于评估细胞死亡事件。使用自体和同源 PRP 与 RP（PRP/RP）研究了 ADSC 的水平迁移方法。所有测定均重复进行三次。流式细胞术和细胞分化表明，II 型胶原酶在收集和冷冻保存后有效分离 ADSC。浓度高达 50 μg.mL-1 的 RP 提取物无细胞毒性作用。浓度为 25 和 50 μg.ml-1 的 PRP 和 RP 联合影响 ADSC 迁移，暴露后第七天完全闭合。此处呈现的结果可能会刺激 ADSC 细胞的增殖，这可能直接或间接地促进组织再生的重建过程。
