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来自[具体物种名称]的[基因名称]基因的功能特性及其在转基因[物种名称]非生物胁迫耐受性中的作用。 (你提供的原文中部分信息缺失,这里是根据格式补充完整后的翻译,实际翻译时请根据准确的原文内容进行)

Functional Characterization of the Gene from and Its Role in Abiotic Stress Tolerance in Transgenic .

作者信息

Ou Chun, Dong Zhiyu, Zheng Xudong, Cheng Wenhui, Chang Ermei, Yao Xiamei

机构信息

Fuyang Normal University-Funan Rural Revitalization Collaborative Technology Service Center, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China.

Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

出版信息

Plants (Basel). 2025 Jan 14;14(2):218. doi: 10.3390/plants14020218.

Abstract

The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing the growth and development of plants to be hindered and even leading to plant death, with abiotic stress hindering the growth and metabolism of plants due to severe uncontrollability. The WHY1 transcription factor plays a critical role in regulating gene expression in plants, influencing chlorophyll biosynthesis, plant growth, and development, as well as responses to environmental stresses. The important role of the gene in regulating plant growth and adaptation to environmental stress has become a hot research topic. However, the mechanism of the gene in under abiotic stress is still unclear. Here, the gene was analyzed bioinformatically using as study material, and the role of the gene against abiotic stress conditions in was verified using transgenic technology. It was found that overexpression of increased seed germination, decreased malondialdehyde accumulation, increased proline content, and delayed the senescence process under salt stress. The expression levels of , , , and were decreased, while the expression levels of , , , and were increased, indicating that overexpression of enhanced the salt stress tolerance of . Furthermore, overexpression also increased drought tolerance in . From the above results, it can be concluded that maintaining high expression levels in the leaves of can improve their environmental adaptability. The results provide a scientific basis for understanding the gene function of the gene of under stress conditions and lay the foundation for further research on the function of the gene.

摘要

世界上极端天气状况的频繁出现给植物生长带来了许多不利因素,导致植物生长发育受阻,甚至造成植物死亡,非生物胁迫因其严重的不可控性阻碍了植物的生长和代谢。WHY1转录因子在调节植物基因表达、影响叶绿素生物合成、植物生长发育以及对环境胁迫的响应中起着关键作用。该基因在调节植物生长和适应环境胁迫方面的重要作用已成为一个热门研究课题。然而,该基因在非生物胁迫下的作用机制仍不清楚。在此,以[具体材料]为研究对象,对该基因进行了生物信息学分析,并利用转基因技术验证了该基因在[具体植物]中对非生物胁迫条件的作用。研究发现,该基因的过表达提高了种子萌发率,降低了丙二醛积累,增加了脯氨酸含量,并在盐胁迫下延缓了衰老过程。[相关基因1]、[相关基因2]、[相关基因3]和[相关基因4]的表达水平降低,而[相关基因5]、[相关基因6]、[相关基因7]和[相关基因8]的表达水平升高,表明该基因的过表达增强了[具体植物]的耐盐胁迫能力。此外,该基因的过表达还提高了[具体植物]的耐旱性。从上述结果可以得出结论,在[具体植物]叶片中维持该基因的高表达水平可以提高其环境适应性。这些结果为理解[具体植物]该基因在胁迫条件下的基因功能提供了科学依据,并为进一步研究该基因的功能奠定了基础。

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