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通过对亚得里亚海中部和东南部进行的综合研究推进硅藻分类学

Advancing the Taxonomy of the Diatom Through an Integrative Study Conducted in the Central and Southeastern Adriatic Sea.

作者信息

Bonačić Tina, Arapov Jasna, Bušelić Ivana, Lepen Pleić Ivana, Milić Roje Blanka, Tomašević Tina, Bužančić Mia, Mladinić Marija, Casabianca Silvia, Penna Antonella, Skejić Sanda, Ninčević Gladan Živana

机构信息

Institute of Oceanography and Fisheries, Šetalište Ivana Meštrovića 63, 21000 Split, Croatia.

Doctoral Study of Biophysics, Faculty of Science, University of Split, Ruđera Boškovića 37, 21000 Split, Croatia.

出版信息

Plants (Basel). 2025 Jan 16;14(2):245. doi: 10.3390/plants14020245.

DOI:10.3390/plants14020245
PMID:39861598
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11768262/
Abstract

The marine diatom genus comprises cosmopolitan phytoplankton species commonly present in the Adriatic Sea. Species within the genus have been of significant concern because they produce domoic acid (DA), which can cause amnesic shellfish poisoning (ASP). In this study, we identified species along the Central and Southeastern Adriatic Sea, where monthly sampling carried out from February 2022 to February 2024 allowed for comprehensive species documentation. species cell cultures isolated from the study areas were morphologically and molecularly analysed. Morphological analyses were performed using a scanning electron microscope (FE-SEM/STEM), while molecular analyses were conducted, targeting the ITS1-5.8S-ITS2, LSU, and L regions, to confirm species identity. This integrative approach led to the identification of eight species: , , , , , , , and . Our findings underscore the value of a combined approach for reliable species identification and contribute to the development of genetic sequence databases that support the advancement of next-generation methods such as metabarcoding. This research emphasises the importance of combined morphological and molecular methods for the differentiation of the cryptic and pseudo-cryptic species.

摘要

海洋硅藻属包含常见于亚得里亚海的世界性浮游植物物种。该属内的物种一直备受关注，因为它们会产生软骨藻酸（DA），可导致失忆性贝类中毒（ASP）。在本研究中，我们在亚得里亚海中部和东南部海域鉴定出了该属物种，从2022年2月至2024年2月进行的月度采样使得能够全面记录物种情况。对从研究区域分离出的该属物种细胞培养物进行了形态学和分子分析。形态学分析使用扫描电子显微镜（场发射扫描电子显微镜/扫描透射电子显微镜）进行，而分子分析则针对ITS1-5.8S-ITS2、大亚基核糖体RNA（LSU）和L区域进行，以确认物种身份。这种综合方法鉴定出了八个物种：[具体物种名称]、[具体物种名称]、[具体物种名称]、[具体物种名称]、[具体物种名称]、[具体物种名称]、[具体物种名称]和[具体物种名称]。我们的研究结果强调了采用综合方法进行可靠物种鉴定的价值，并有助于开发支持元条形码等下一代方法发展的遗传序列数据库。这项研究强调了形态学和分子方法相结合对于区分隐种和拟隐种的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c3/11768262/4bdb20169ec4/plants-14-00245-g014.jpg
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