Beas-Guzmán Oscar F, Cabrera-Licona Ariana, Hernández-Fuentes Gustavo A, Ceballos-Magaña Silvia G, Guzmán-Esquivel José, De-León-Zaragoza Luis, Ramírez-Flores Mario, Diaz-Martinez Janet, Garza-Veloz Idalia, Martínez-Fierro Margarita L, Rodríguez-Sanchez Iram P, Ceja-Espíritu Gabriel, Meza-Robles Carmen, Cervantes-Kardasch Víctor H, Delgado-Enciso Iván
Department of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, Mexico.
State Cancerology Institute of Colima, Health Services of the Mexican Social Security Institute for Welfare (IMSS-BIENESTAR), Colima 28085, Mexico.
Pharmaceutics. 2024 Dec 24;17(1):2. doi: 10.3390/pharmaceutics17010002.
: , or star fruit, is a shrub known for its medicinal properties, especially due to bioactive metabolites identified in its roots and fruit with anti-cancer activity. However, the biological effects of its leaves remain unexplored. This study aimed to assess the effects of ethanolic extract from leaves on triple-negative breast cancer (TNBC), an aggressive subtype lacking specific therapy. : Phytochemical analysis and HPLC profile and additional cell line evaluation employing MDA-MB-231 were carried out. : Phytochemical screening revealed that the ethanolic extract was rich in flavonoids, saponins, and steroids, demonstrating an antioxidant capacity of 45%. H NMR analysis indicated the presence of flavonoids, terpenes, and glycoside-like compounds. Cell viability assays showed a concentration-dependent decrease in viability, with an IC value of 20.89 μg/mL at 48 h. Clonogenic assays indicated significant inhibition of replicative immortality, with only 2.63% survival at 15 μg/mL. Migration, assessed through a wound healing assay, was reduced to 3.06% at 100 μg/mL, with only 16.23% of cells remaining attached. An additive effect was observed when combining lower concentrations of the extract with doxorubicin, indicating potential synergy. : These results suggest that the ethanolic extract of leaves contains metabolites with anti-cancer activity against TNBC cells, supporting further research into their bioactive compounds and therapeutic potential.
杨桃是一种灌木，以其药用特性而闻名，特别是因其根和果实中鉴定出具有抗癌活性的生物活性代谢物。然而，其叶子的生物学效应尚未得到探索。本研究旨在评估杨桃叶乙醇提取物对三阴性乳腺癌（TNBC）的影响，TNBC是一种缺乏特异性治疗方法的侵袭性亚型。开展了植物化学分析、高效液相色谱分析以及使用MDA-MB-231细胞系的额外评估。植物化学筛选显示，乙醇提取物富含黄酮类化合物、皂苷和甾体，抗氧化能力为45%。核磁共振氢谱分析表明存在黄酮类化合物、萜类化合物和糖苷类化合物。细胞活力测定显示活力呈浓度依赖性下降，48小时时IC值为20.89μg/mL。克隆形成试验表明对复制永生有显著抑制作用，15μg/mL时存活率仅为2.63%。通过伤口愈合试验评估的迁移能力在100μg/mL时降至3.06%，仅有16.23%的细胞仍附着。将较低浓度的提取物与多柔比星联合使用时观察到相加效应，表明可能存在协同作用。这些结果表明，杨桃叶乙醇提取物含有对TNBC细胞具有抗癌活性的代谢物，支持对其生物活性化合物和治疗潜力进行进一步研究。
