Lian Qun, Zhang Yingying, Zhang Jinzhe, Peng Zhen, Wang Weilun, Du Miru, Li Hongbo, Zhang Xinyan, Cheng Lin, Du Ran, Zhou Zijian, Yang Zhenqiang, Xin Guohui, Pu Yuanyuan, Feng Zhiwen, Wu Qian, Xuanyuan Guochao, Bai Shunbuer, Hu Rong, Negrão Sónia, Bryan Glenn J, Bachem Christian W B, Zhou Yongfeng, Zhang Ruofang, Shang Yi, Huang Sanwen, Lin Tao, Qi Jianjian
Inner Mongolia Potato Engineering and Technology Research Center, Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010021, China; National Key Laboratory of Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; School of Biology and Environmental Science, University College Dublin, Dublin, Ireland.
Inner Mongolia Potato Engineering and Technology Research Center, Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
Mol Plant. 2025 Apr 7;18(4):570-589. doi: 10.1016/j.molp.2025.01.016. Epub 2025 Jan 23.
Hybrid potato breeding based on diploid inbred lines is transforming the way of genetic improvement of this staple food crop, which requires a deep understanding of potato domestication and differentiation. In the present study, we resequenced 314 diploid wild and landrace accessions to generate a variome map of 47,203,407 variants. Using the variome map, we discovered the reshaping of tuber transcriptome during potato domestication, characterized genome-wide differentiation between landrace groups Stenotomum and Phureja. We identified a jasmonic acid biosynthetic gene possibly affecting the tuber dormancy period. Genome-wide association studies revealed a UDP-glycosyltransferase gene for the biosynthesis of anti-nutritional steroidal glycoalkaloids (SGAs), and a Dehydration Responsive Element Binding (DREB) transcription factor conferring increased average tuber weight. In addition, genome similarity and group-specific SNP analyses indicated that tetraploid potatoes originated from the diploid Solanum tuberosum group Stenotomum. These findings shed light on the evolutionary trajectory of potato domestication and improvement, providing a solid foundation for advancing hybrid potato-breeding practices.
基于二倍体自交系的杂交马铃薯育种正在改变这种主食作物的遗传改良方式，这需要深入了解马铃薯的驯化和分化过程。在本研究中，我们对314份二倍体野生和地方品种材料进行了重测序，以生成包含47,203,407个变异的变异组图谱。利用该变异组图谱，我们发现了马铃薯驯化过程中块茎转录组的重塑，描绘了地方品种窄刀薯组（Stenotomum）和光果薯组（Phureja）之间全基因组的分化。我们鉴定出一个可能影响块茎休眠期的茉莉酸生物合成基因。全基因组关联研究揭示了一个参与抗营养甾体糖苷生物碱（SGA）生物合成的尿苷二磷酸糖基转移酶基因，以及一个能增加块茎平均重量的脱水响应元件结合（DREB）转录因子。此外，基因组相似性和群体特异性单核苷酸多态性分析表明，四倍体马铃薯起源于二倍体窄刀薯组（Solanum tuberosum group Stenotomum）。这些发现揭示了马铃薯驯化和改良的进化轨迹，为推进杂交马铃薯育种实践提供了坚实基础。
