Grosberg A Iu, Zhestkov A V
Biofizika. 1985 Mar-Apr;30(2):233-8.
A diagram of the states of single long rigid DNA macromolecule in solution of short flexible polymer was plotted. It has been shown that the compacting effect of the polymer solvent on DNA can be interpreted as hydrostatic pressure of coils gas on impermeable "walls" of the DNA globule. Conditions were found for the existence of compact (globular) states in the form of a sphere (stable for a very long DNA) and tore (for a relatively short one). Possibility of intramolecular liquidcrystalline orderliness of DNA segments in a globule was analyzed. Regions of realization of both regimes were studied: when compression with an added polymer was equalized by the forces of steric repulsion of DNA segments and when it was balanced by the elasticity of the DNA chain bendings.
绘制了短柔性聚合物溶液中单个长刚性DNA大分子状态的示意图。结果表明,聚合物溶剂对DNA的压缩作用可解释为卷曲气体对DNA球状体不可渗透“壁”的流体静压力。发现了以球体形式(对非常长的DNA稳定)和撕裂形式(对相对较短的DNA)存在致密(球状)状态的条件。分析了球状体中DNA片段分子内液晶有序性的可能性。研究了两种状态的实现区域:当添加聚合物的压缩与DNA片段的空间排斥力平衡时,以及当它由DNA链弯曲的弹性平衡时。