使用交替双侧感觉刺激减轻小鼠酒精诱导的条件性位置偏爱实验方案。
Protocol for alleviating alcohol-induced conditioned place preference in mice using alternating bilateral sensory stimulation.
作者信息
Lei Jie, Li Tongxia, Cui Chi, Ren Kun, Shi Yulong, Yao Yibo, Luo Gangan, Li Ming, Peng Xiang, Yang Xueke, Yang Jian, Zhang Pei, Tian Bo
机构信息
Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Institute for Brain Research, Huazhong University of Science and Technology, Wuhan 430030, China; Key Laboratory of Neurological Diseases, Ministry of Education, Wuhan 430030, China.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103596. doi: 10.1016/j.xpro.2025.103596. Epub 2025 Jan 24.
Alternating bilateral sensory stimulation (ABS) is a clinical physical therapy technique effective in treating post-traumatic stress disorder (PTSD). However, its utilization in treating conditions beyond PTSD remains limited. Here, we present a protocol to reduce ethanol-induced conditioned place preference (CPP) using 4 Hz ABS. We describe steps for the assembly of ABS apparatus, CPP training, and ABS treatment. This protocol has been validated in male mice and can be used to explore the mechanism behind the therapeutic effect of ABS. For complete details on the use and execution of this protocol, please refer to Lei et al..
交替双侧感觉刺激（ABS）是一种在治疗创伤后应激障碍（PTSD）方面有效的临床物理治疗技术。然而，其在治疗PTSD以外病症中的应用仍然有限。在此，我们提出一种使用4赫兹ABS来减少乙醇诱导的条件性位置偏爱（CPP）的方案。我们描述了ABS装置组装、CPP训练和ABS治疗的步骤。该方案已在雄性小鼠中得到验证，可用于探索ABS治疗效果背后的机制。有关该方案使用和执行的完整详细信息，请参考 Lei等人的研究。
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