雌激素处理后去卵巢大鼠子宫中基质蛋白的比较。

Comparison of media proteins from ovariectomized rat uteri following estrogen treatment.

作者信息

Komm B S, Keeping H S, Sabogal G, Lyttle C R

出版信息

Biol Reprod. 1985 Mar;32(2):443-50. doi: 10.1095/biolreprod32.2.443.

DOI:10.1095/biolreprod32.2.443

Abstract

Ovariectomized rats were treated with estradiol for 3 days after which their uteri were incubated in vitro and radioactive media proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Media were also chromatographed on G-25 Sephadex Blue Sepharose columns to isolate subsets of proteins. The results demonstrate that two proteins are consistently increased following estrogen treatment. These proteins have molecular weights of 104,000 and 65,000. Neither protein binds to Blue Sepharose to a great extent. The use of the protein synthesis inhibitors, emetine and actinomycin D, demonstrates that the proteins are synthesized de novo. These two proteins may serve as markers for genomic response to estradiol in the rat uterus.

摘要

对去卵巢大鼠用雌二醇治疗3天，之后将其子宫进行体外培养，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析放射性培养基蛋白。培养基还在G-25葡聚糖凝胶蓝琼脂糖柱上进行层析以分离蛋白质亚群。结果表明，雌激素治疗后两种蛋白质持续增加。这些蛋白质的分子量分别为104,000和65,000。两种蛋白质都不太能与蓝琼脂糖结合。使用蛋白质合成抑制剂放线菌酮和放线菌素D表明这些蛋白质是重新合成的。这两种蛋白质可能作为大鼠子宫中对雌二醇基因组反应的标志物。