子宫雌激素诱导蛋白：与卵清蛋白的物理及免疫学比较
Uterine estrogen-induced protein: physical and immunological comparison with ovalbumin.
作者信息
Katzenellenbogen B S, Williams L B
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1281-5. doi: 10.1073/pnas.71.4.1281.
The physical and immunological properties of the estrogen-induced protein from rat uterus are compared with those of ovalbumin from chick oviduct. Both proteins are induced by estrogen and are under estrogenic regulation. Electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gels and elution behavior on Sephadex G-100 column chromatography indicate that the uterine protein has a molecular weight of about 42,000, similar to that of ovalbumin, and likewise is composed of only one subunit. On isoelectric focusing in polyacrylamide gels, both proteins focus at a pH of 4.6. The uterine protein, in addition, shows a component with an isoelectric point of 5.1. Despite considerable similarity in the physical properties of the uterine protein and ovalbumin, there is no significant crossreactivity of anti-ovalbumin gamma globulin with the uterine protein, implying that these two estrogen-regulated proteins are immunologically distinct.
将大鼠子宫中雌激素诱导蛋白的物理和免疫学特性与鸡输卵管中的卵清蛋白的特性进行了比较。这两种蛋白均由雌激素诱导产生，并受雌激素调节。在十二烷基硫酸钠-聚丙烯酰胺凝胶上的电泳迁移率以及在葡聚糖G-100柱色谱上的洗脱行为表明，子宫蛋白的分子量约为42,000，与卵清蛋白相似，同样仅由一个亚基组成。在聚丙烯酰胺凝胶中进行等电聚焦时，两种蛋白均聚焦于pH 4.6处。此外，子宫蛋白还显示出一个等电点为5.1的组分。尽管子宫蛋白和卵清蛋白在物理性质上有相当大的相似性，但抗卵清蛋白γ球蛋白与子宫蛋白之间没有明显的交叉反应，这意味着这两种受雌激素调节的蛋白在免疫学上是不同的。
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