Saweres-Argüelles C, Sánchez-Calvo A, Serrano-Pertierra E, Matos M, Blanco-López M C
Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain.
Department of Biochemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006, Oviedo, Spain.
Anal Chim Acta. 2025 Feb 22;1340:343597. doi: 10.1016/j.aca.2024.343597. Epub 2024 Dec 28.
The COVID-19 outbreak was an important turning point in the development of a new generation of biosensing technologies. The synergistic combination of an immunochromatographic test (lateral flow immunoassays, LFIA) and signal transducers provides enhanced sensitivity and the ability to quantify in the rapid tests. This is possible due to the variety of nanoparticles that can be used as reporter labels. In this review, we first present an overview on the principles of a LFIA and its different formats. We analyze cutting-edge work on these platforms based on different types of nanoparticles used as labels and on the highly sensitive transducers to which they can be coupled. The works discussed herein have a beneficial impact on the fields of clinical analysis, food safety or environmental control, thus highlighting the relevance of the biosensors. Last, we provide insights into the barriers that need to be overcome when designing laboratory prototypes accessible to the society.
新冠疫情是新一代生物传感技术发展中的一个重要转折点。免疫层析检测（侧向流动免疫分析，LFIA）与信号传感器的协同结合提高了灵敏度，并具备在快速检测中进行定量分析的能力。这得益于多种可作为报告标签的纳米颗粒。在本综述中，我们首先概述了LFIA的原理及其不同形式。我们基于用作标签的不同类型纳米颗粒以及与之耦合的高灵敏度传感器，分析了这些平台上的前沿研究工作。本文讨论的研究成果对临床分析、食品安全或环境控制领域具有有益影响，从而凸显了生物传感器的重要性。最后，我们深入探讨了在设计面向社会大众的实验室原型时需要克服的障碍。
