Yang Chengjie, Zhou Yurui, Fan Chuan, Wang Honglu, Xu Jiaxin, Wu Zhicheng, Song Yan, Hu Yanbin, Tian Meng, Liu Guodong
School of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, China.
Mikrochim Acta. 2025 Jun 18;192(7):431. doi: 10.1007/s00604-025-07260-4.
The first visual lateral flow immunoassay (LFIA) is reported using gold nanotriangles (AuNTs) as labeling materials for high-sensitivity alpha-fetoprotein (AFP) detection in serum. Compared with traditional gold nanoparticles, the use of anisotropic AuNTs significantly reduces the detection limit of AFP due to larger surface area and strong localized surface plasmon resonance, which can reach 5 ng/mL in serum samples with naked eyes. Under optimized conditions, this method demonstrates excellent accuracy and reproducibility in both buffer and serum systems. More importantly, the direct use of gold nanotriangles as labeling materials has the advantages of simple synthesis and strong controllability compared with complex composite labeling materials. This work pioneers the application of AuNTs in LFIA, bridging the gap between simplicity (visual readout) and high sensitivity, and provides a scalable platform for point-of-care diagnostics and is expected to have commercial application prospects.
首次报道了使用金纳米三角形（AuNTs）作为标记材料的可视化侧向流动免疫分析（LFIA），用于血清中高灵敏度甲胎蛋白（AFP）的检测。与传统金纳米颗粒相比，由于具有更大的表面积和强烈的局域表面等离子体共振，使用各向异性的AuNTs显著降低了AFP的检测限，在血清样本中肉眼检测限可达5 ng/mL。在优化条件下，该方法在缓冲液和血清体系中均表现出优异的准确性和重现性。更重要的是，与复杂的复合标记材料相比，直接使用金纳米三角形作为标记材料具有合成简单和可控性强的优点。这项工作开创了AuNTs在LFIA中的应用，弥合了简单性（视觉读数）和高灵敏度之间的差距，并为即时诊断提供了一个可扩展的平台，有望具有商业应用前景。
