Ádám Bálint Árpád, Spátay Sára, Jávor Bálint, László Szabolcs, Illés Levente, Fürjes Péter, Tóth Tünde, Huszthy Péter, Golcs Ádám
Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Szent Gellért Tér 4, 1111, Budapest, Hungary.
Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre U. 9, 1092, Budapest, Hungary.
Sci Rep. 2025 Jan 25;15(1):3238. doi: 10.1038/s41598-024-85040-5.
Microtiter-plate-based systems are unified platforms of high-throughput experimentation (HTE). These polymeric devices are used worldwide on a daily basis-mainly in the pharmaceutical industry-for parallel syntheses, reaction optimization, various preclinical studies and high-throughput screening methods. Accordingly, laboratory automation today aims to handle these commercially available multiwell plates, making developments focused on their modifications a priority area of modern applied research. We performed the covalent functionalization of the porous PVDF-membrane of microtiter filter plates as the essence of conventional and common sandwich plate systems by introducing a generalizable method. After surface-activation of the indifferent membrane polymer, customizable functionalization becomes feasible by covalently attached monofunctional molecular linkers. The study was designed with future adaptability, and thus, industrially widespread atmospheric plasma and two different chemical treatments were investigated and compared in terms of practical implementation, polarization effects, extent of labeling, effects on morphology and porosity as well as on permeability. For critical comparison, contact angle measurements, surface ATR-FTIR, H-NMR, F-NMR, UV-Vis spectroscopy, scanning electron microscopy and permeability tests were used.
基于微量滴定板的系统是高通量实验（HTE）的统一平台。这些聚合物装置每天在全球范围内使用，主要用于制药行业，进行平行合成、反应优化、各种临床前研究和高通量筛选方法。因此，当今的实验室自动化旨在处理这些商用多孔板，使得专注于其改进的开发成为现代应用研究的一个优先领域。我们通过引入一种通用方法，对微量滴定滤板的多孔聚偏氟乙烯膜进行共价功能化，这是传统和常见夹心板系统的核心。在对惰性膜聚合物进行表面活化后，通过共价连接单功能分子连接体实现可定制的功能化成为可能。该研究设计具有未来适应性，因此，对工业上广泛使用的常压等离子体和两种不同的化学处理方法在实际应用、极化效应、标记程度、对形态和孔隙率以及渗透性的影响方面进行了研究和比较。为了进行关键比较，使用了接触角测量、表面衰减全反射傅里叶变换红外光谱（ATR-FTIR）、氢核磁共振（H-NMR）、氟核磁共振（F-NMR）、紫外可见光谱（UV-Vis）、扫描电子显微镜和渗透性测试。
