Badenoch D F, Fowler C G, Evans P R, Lowrey P A
Br J Urol. 1985 Apr;57(2):230-2. doi: 10.1111/j.1464-410x.1985.tb06431.x.
In this study we have adapted a well tried and sensitive fluorometric assay for DNA that uses m-diamino benzoic acid (DABA) for its reaction with deoxyribose which is cleaved by perchloric acid from DNA. We have confirmed that this is easily measured in human semen. The method has been used on 200 samples of semen from patients attending the Infertility Clinic and men attending before and after vasectomy to give a wide range of sperm density. The objective DNA estimation has been compared with sperm density measurement made by an experienced laboratory observer using the Makler chamber and making his observations in ignorance of the DNA estimations. There was good correlation between DNA concentration and sperm density. The experimental accuracy of the two methods was found to be comparable. The advantage of the method is its objectivity. However, its disadvantages are its cost in terms of time and material and it cannot be used to confirm post-vasectomy azoospermia because the method also measures DNA in leucocytes.
在本研究中，我们采用了一种经过充分验证且灵敏的DNA荧光测定法，该方法使用间二氨基苯甲酸（DABA）与脱氧核糖反应，脱氧核糖由高氯酸从DNA中裂解出来。我们已证实，这在人类精液中易于测量。该方法已应用于200份来自不育门诊患者以及输精管结扎术前和术后男性的精液样本，以提供广泛的精子密度范围。客观的DNA估计值已与由经验丰富的实验室观察员使用马克勒计数池进行的精子密度测量结果进行比较，且该观察员在不知DNA估计值的情况下进行观察。DNA浓度与精子密度之间存在良好的相关性。发现这两种方法的实验准确性相当。该方法的优点是其客观性。然而，其缺点是在时间和材料方面成本较高，并且不能用于确认输精管结扎术后无精子症，因为该方法也会测量白细胞中的DNA。
