Koyano Yuki, Fujinoki Masakatsu
Research Laboratory of Laboratory Animals, Research Center for Laboratory Animals, Comprehensive Research Facilities for Advanced Medical Science, School of Medicine, Dokkyo Medical University, Tochigi 321-0293, Japan.
J Reprod Dev. 2025 Apr 14;71(2):85-92. doi: 10.1262/jrd.2024-078. Epub 2025 Jan 25.
The neurotransmitter, 5-hydroxytriptamine (5-HT), is well known. Furthermore, it enhances the acrosome reaction, hyperactivation, and in vitro fertilization (IVF) success in hamsters and mice. In the present study, we examined whether 5-HT enhances hyperactivation and increases IVF success in rats. When rat sperm was exposed to 5-HT, hyperactivation was significantly enhanced. Only the 5-HT receptor agonists significantly enhanced hyperactivation. Additionally, both 5-HT and 5-HT receptor agonists significantly increase the success of IVF. These results suggested that 5-HT increases IVF success by enhancing hyperactivation and effects of 5-HT are associated with the 5-HT receptor. Therefore, in rats, 5-HT enhances capacitation and the 5-HT receptor is the key molecule for capacitation.
神经递质5-羟色胺（5-HT）广为人知。此外，它能增强仓鼠和小鼠的顶体反应、超激活以及体外受精（IVF）成功率。在本研究中，我们检测了5-HT是否能增强大鼠的超激活并提高IVF成功率。当大鼠精子暴露于5-HT时，超激活显著增强。只有5-HT受体激动剂能显著增强超激活。此外，5-HT和5-HT受体激动剂均能显著提高IVF成功率。这些结果表明，5-HT通过增强超激活来提高IVF成功率，且5-HT的作用与5-HT受体相关。因此，在大鼠中，5-HT增强获能，且5-HT受体是获能的关键分子。
