Zhang Bowen, Ni Weixiang, Cai Qinglong, Ji Huitao, Du Junhao, Hong Weixuan, Fang Junwei, Wang Lie, Qi Yafeng, Xiao Chunhong
Department of General Surgery, Dongfang Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University/Fuzong Clinical Medical College of Fujian Medical University/College of Integrative Medicine Fujian University of Traditional Chinese Medicine/The 900th Hospital of Joint Logistics Support Force, PLA, Fuzhou, China.
Animal Model Exp Med. 2025 Mar;8(3):421-428. doi: 10.1002/ame2.12517. Epub 2025 Jan 26.
The aim of the study was to explore a feasible method for alleviating limb ischemia-reperfusion injury (LI/RI) through the use of a high-concentration citrate solution (HC-A solution) for limb perfusion (LP).
Eighteen pigs were divided into three groups: the Sham group, LI/RI group, and HCA group. The Sham group underwent exposure of the iliac artery and vein. The LI/RI group underwent tourniquet placement and clamping of the iliac artery and vein to simulate LI/RI. The HCA group received HC-A solution LP for 30 min through the left iliac artery below the level of blood flow occlusion based on the LI/RI group. Oxidative stress markers and inflammatory response markers were compared among the three groups.
Compared to the LI/RI group, the HCA group showed significantly lower levels of serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), and significantly greater activities of serum superoxide dismutase (SOD) (p < 0.05). There were no significant differences in serum interleukin-6 (IL-6) or in muscle MDA, SOD, TNF-α, and IL-6 between the HCA group and the LI/RI group (p > 0.05). Compared to the LI/RI group, MDA, TNF-α, and IL-6 levels in the liver were significantly lower in the HCA group (p < 0.05), while SOD activities were not significantly different (p > 0.05). Histopathological examination revealed reduced skeletal muscle and liver damage in the HCA group compared to the LI/RI group.
HC-A solution LP can alleviate liver damage caused by LI/RI in pigs.
本研究的目的是探索一种通过使用高浓度柠檬酸盐溶液（HC-A溶液）进行肢体灌注（LP）来减轻肢体缺血再灌注损伤（LI/RI）的可行方法。
将18头猪分为三组：假手术组、LI/RI组和HCA组。假手术组进行髂动脉和静脉暴露。LI/RI组进行止血带放置并夹闭髂动脉和静脉以模拟LI/RI。HCA组在LI/RI组的基础上，通过在血流阻断水平以下的左髂动脉接受HC-A溶液LP 30分钟。比较三组的氧化应激标志物和炎症反应标志物。
与LI/RI组相比，HCA组血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平显著降低，血清超氧化物歧化酶（SOD）活性显著升高（p<0.05）。HCA组与LI/RI组之间血清白细胞介素-6（IL-6）或肌肉MDA、SOD、TNF-α和IL-6无显著差异（p>0.05）。与LI/RI组相比，HCA组肝脏中的MDA、TNF-α和IL-6水平显著降低（p<0.05），而SOD活性无显著差异（p>0.05）。组织病理学检查显示，与LI/RI组相比，HCA组骨骼肌和肝脏损伤减轻。
HC-A溶液LP可减轻猪LI/RI所致的肝脏损伤。
