Babinskas Justinas, Matijošytė Inga
Sector of Applied Biocatalysis, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio ave. 7, Vilnius, Lithuania, LT-10257.
Chembiochem. 2025 Apr 1;26(7):e202400939. doi: 10.1002/cbic.202400939. Epub 2025 Feb 5.
Enzyme functional analysis is a multifaceted process that can be used for various purposes, such as screening for specific activities, as well as developing, optimising, and validating processes or final products. Functional analysis methods are crucial for assessing enzyme performance and catalytic properties. Laccase, a well-known blue multi-copper oxidase, holds immense potential in diverse industries such as pharmaceuticals, paper and pulp, food and beverages, textiles, and biorefineries due to its clean oxidation process and versatility in handling a wide range of substrates. Despite its prominence, the use of laccase encounters challenges in selecting appropriate functional analysis substrates and methods. This review delves into the substrates utilised in qualitative and quantitative techniques for laccase activity analysis. Although laccase catalyses mono-electron oxidation of aromatic hydroxyl, amine, and thiol compounds efficiently, using molecular oxygen as an electron acceptor, the review identifies limitations in the specificity of the commonly employed substrates, concerns regarding the stability of certain compounds and highlights potential strategies.
酶功能分析是一个多方面的过程，可用于各种目的，如筛选特定活性，以及开发、优化和验证工艺或最终产品。功能分析方法对于评估酶的性能和催化特性至关重要。漆酶是一种著名的蓝色多铜氧化酶，由于其清洁的氧化过程以及处理多种底物的通用性，在制药、造纸和纸浆、食品和饮料、纺织品以及生物精炼等不同行业具有巨大潜力。尽管漆酶很突出，但在选择合适的功能分析底物和方法时，漆酶的使用面临挑战。本综述深入探讨了用于漆酶活性分析的定性和定量技术中所使用的底物。尽管漆酶能有效地催化芳香族羟基、胺和硫醇化合物的单电子氧化反应，并以分子氧作为电子受体，但该综述指出了常用底物特异性方面的局限性、某些化合物稳定性方面的问题，并强调了潜在的策略。
