Shah Sakhawat, Shi Chun-Mei, Elgizawy Karam Khamis, Yan Wen-Han, Wu Gang, Wang Xiao-Ping, Yang Feng-Lian
Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan, China.
Front Cell Dev Biol. 2025 Jan 10;12:1507725. doi: 10.3389/fcell.2024.1507725. eCollection 2024.
Spermatogenesis in Lepidoptera holds significant importance due to its unique process of dichotomous spermatogenesis, yielding eupyrene and apyrene spermatozoa through a complex molecular mechanism. While E3 ubiquitin ligases are known to play vital roles in spermatogenesis across various processes, their functions in dichotomous spermatogenesis remain less known. We utilized the RNAi, biochemical and microscopic procedures to unravel the function of in dichotomous spermatogenesis of adult . In . ligase predominantly expressed in adult tissues. Knockdown of leads to disruptions in testes and sperm morphology, affecting the structure of eupyrene and apyrene sperm bundles and causing defective ultrastructure in eupyrene sperm. This disruption results in a reduction in the number of dichotomous sperms and significantly reduces their motility. Moreover, knockdown inhibits the transfer and motility of dichotomous sperm, impacting spermatophore formation in females and ultimately reducing egg production. Understanding the role of is not only crucial for comprehending the complex processes involved in dichotomous spermatogenesis and fertilization but also provides an avenue for sustainable pest control management.
鳞翅目昆虫的精子发生具有重要意义，因为其独特的二分法精子发生过程通过复杂的分子机制产生真核精子和无核精子。虽然已知E3泛素连接酶在精子发生的各个过程中发挥重要作用，但其在二分法精子发生中的功能仍鲜为人知。我们利用RNA干扰、生化和显微镜技术来揭示其在成年昆虫二分法精子发生中的功能。在成年昆虫中，连接酶主要在成年组织中表达。敲低该连接酶会导致睾丸和精子形态紊乱，影响真核精子束和无核精子束的结构，并导致真核精子的超微结构缺陷。这种紊乱导致二分法精子数量减少，并显著降低其活力。此外，敲低该连接酶会抑制二分法精子的转移和活力，影响雌性受精囊的形成，最终减少产卵量。了解该连接酶的作用不仅对于理解二分法精子发生和受精所涉及的复杂过程至关重要，而且为可持续害虫控制管理提供了一条途径。
