Bogolyubov Dmitry S, Chistyakova Ludmila V, Sokolova Yuliya Y, Goodkov Andrew V
Laboratory of Cell Morphology, Institute of Cytology of the Russian Academy of Sciences, St. Petersburg, Russia.
Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia.
J Eukaryot Microbiol. 2025 Jan-Feb;72(1):e70000. doi: 10.1111/jeu.70000.
The genus Pelomyxa includes 15 species of anaerobic Archamoebae with remarkable diverse nucleoplasm morphology. Nuclear structures, like chromatin and nucleoli, of several members of the genus was previously identified only based on their ultrastructural similarity to typical structures of somatic cells of higher eukaryotes. Here, we explored an easy-to-use, one-step intravital staining method with DAPI and pyronin to distinguish between DNA and RNA structures in nuclei of unfixed cells of Pelomyxa belevskii and P. stagnalis significantly varying by nuclear organization. Our method can be used for rapid diagnosis of DNA and RNA-containing structures in species with complex nuclear organization.
多核变形虫属包括15种厌氧原始阿米巴，其核质形态显著多样。该属几个成员的核结构，如染色质和核仁，此前仅根据它们与高等真核生物体细胞典型结构的超微结构相似性来识别。在这里，我们探索了一种易于使用的一步活体染色方法，使用4',6-二脒基-2-苯基吲哚（DAPI）和派洛宁来区分贝氏多核变形虫和停滞多核变形虫未固定细胞细胞核中的DNA和RNA结构，这两种变形虫的核组织有显著差异。我们的方法可用于快速诊断核组织复杂的物种中含DNA和RNA的结构。
