Jorgensen J H
Chemioterapia. 1985 Feb;4(1):7-13.
Several antimicrobial susceptibility test methods in current use including the agar dilution, broth microdilution, Kirby-Bauer, and rapid automated instrument test methods have been criticized for failing to detect resistance due to chromosomally mediated beta-lactamases of certain Gram-negative bacteria. The older broth macrodilution test may be the most reliable method for recognition of low numbers of drug resistant mutants. In vitro induction of beta-lactamases by drugs such as cefoxitin may serve only to reaffirm the presence of the chromosomal gene in those species known to possess this mechanism of drug resistance.
目前使用的几种抗菌药物敏感性试验方法，包括琼脂稀释法、肉汤微量稀释法、 Kirby-Bauer法和快速自动化仪器检测方法，都因无法检测某些革兰氏阴性菌染色体介导的β-内酰胺酶导致的耐药性而受到批评。较老的肉汤常量稀释试验可能是识别少量耐药突变体最可靠的方法。头孢西丁等药物在体外诱导β-内酰胺酶，可能仅用于再次确认已知具有这种耐药机制的物种中染色体基因的存在。
