Zhang Yuning, Lv Yanbing, Chen Xinxin, Ye Yingli, Yu Shenping, Li Ning, Yao Hongtao, Wang Zhizeng, Feng Qitong, Wu Ruili, Li Lin Song
Key Lab for Special Functional Materials of Ministry of Education, and School of Nanoscience and Materials Engineering, Henan University, 475004, Kaifeng, China.
Beijing Lumitest biotechnology co., ltd, 100191, Beijing, China.
Biosens Bioelectron. 2025 Apr 1;273:117187. doi: 10.1016/j.bios.2025.117187. Epub 2025 Jan 23.
Influenza A virus (IAV) and influenza B virus (IBV) with similar symptoms of infection caused a serious disease burden and economic losses in annual epidemic season, so it is important to quickly and accurately detect and distinguish between IAV and IBV during influenza season. Herein, the quantum dot microspheres (QDMS) were synthesized and applied to lateral flow immunoassays (LFIA), and a point-of-care (POC) biosensor that can discriminately and simultaneously diagnose IAV and IBV within 10 min was established. A double-sandwich QDMS nanotags was synthesized by immobilizing hydrophobic quantum dots (QDs) with chemical bonding method on a silica sphere template with an outer silica shell protection showed excellent stability and high fluorescence. The highly sensitive quantitative detection is achieved with the tailored hand-hold detector. The limit of detection (LOD) of the LFIA based QDMS for the simultaneous detection of IAV and IBV were 0.080 ng/mL and 0.096 ng/mL, respectively. The QDMS-LFIA system has excellent specificity and stability for quantitative detection, especially showing good accuracy for clinical samples. Furthermore, the visualization sensitivity of the low-cost biosensor was improved by nearly an order of magnitude than gold nanoparticles (AuNP)-LFIA within shorter detection time. Given the excellent performance, our proposed QDMS-LFIA biosensor can potentially be applied to the POC sensitive discriminate and simultaneous detection of IAV and IBV in epidemic season.
甲型流感病毒（IAV）和乙型流感病毒（IBV）感染症状相似，在每年的流行季节造成了严重的疾病负担和经济损失，因此在流感季节快速准确地检测和区分IAV和IBV非常重要。在此，合成了量子点微球（QDMS）并将其应用于侧向流动免疫分析（LFIA），建立了一种能够在10分钟内同时鉴别诊断IAV和IBV的即时检测（POC）生物传感器。通过化学键合法将疏水性量子点（QDs）固定在具有外部二氧化硅壳保护的二氧化硅球模板上，合成了双夹心QDMS纳米标签，其表现出优异的稳定性和高荧光性。使用定制的手持式检测器实现了高灵敏度的定量检测。基于QDMS的LFIA同时检测IAV和IBV的检测限（LOD）分别为0.080 ng/mL和0.096 ng/mL。QDMS-LFIA系统在定量检测方面具有优异的特异性和稳定性，对临床样本尤其显示出良好的准确性。此外，在更短的检测时间内，这种低成本生物传感器的可视化灵敏度比金纳米颗粒（AuNP）-LFIA提高了近一个数量级。鉴于其优异的性能，我们提出的QDMS-LFIA生物传感器有可能应用于流感流行季节IAV和IBV的即时检测灵敏鉴别和同时检测。
