Yasin Najwa Mat, Polanska Monika, Verbeken Kim, Van Impe Jan F M, Akkermans Simen
BioTeC+ - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders De Smetstraat 1, 9000 Gent, Belgium; Faculty of Ocean Engineering, Universiti Malaysia Terengganu (UMT), 21030 Terengganu, Malaysia.
BioTeC+ - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Gebroeders De Smetstraat 1, 9000 Gent, Belgium.
Bioresour Technol. 2025 Mar;420:132114. doi: 10.1016/j.biortech.2025.132114. Epub 2025 Jan 25.
Environmental pollution from packaging, has led to a need for sustainable alternatives. This study investigates the biodegradation of polylactic acid (PLA) by Amycolatopsis orientalis and Amycolatopsis thailandensis after thermal and thermal-alkaline pretreatments. The biodegradation was assessed based on weight loss, CO evolution, carbon balance analysis and scanning electron microscopy (SEM). The analysis showed that pretreatment at 37 °C for 8 h provided effective enhancement of the biodegradation performance. Combining thermal pretreatment with alkaline conditions led to chemical degradation of PLA, but is less suitable as a pretreatment for biodegradation. It was also demonstrated that the mineralization rate over a two-week period was higher following thermal than thermal-alkaline pretreatment. SEM confirmed improved biodegradation as illustrated by increased surface roughness. These findings suggest that thermal pretreatment at 37 °C for 8 h is the most effective strategy for enhancing PLA biodegradation by Amycolatopsis spp., promoting a sustainable approach to plastic waste management.
包装造成的环境污染，引发了对可持续替代方案的需求。本研究调查了东方拟无枝菌酸菌和泰国拟无枝菌酸菌在热预处理和热碱预处理后对聚乳酸（PLA）的生物降解情况。基于重量损失、二氧化碳释放、碳平衡分析和扫描电子显微镜（SEM）对生物降解进行了评估。分析表明，在37°C下预处理8小时可有效提高生物降解性能。将热预处理与碱性条件相结合会导致PLA发生化学降解，但不太适合作为生物降解的预处理方法。研究还表明，在两周时间内，热预处理后的矿化率高于热碱预处理。SEM证实了生物降解的改善，表现为表面粗糙度增加。这些发现表明，在37°C下预处理8小时是增强拟无枝菌酸菌对PLA生物降解的最有效策略，推动了塑料废物管理的可持续方法。
