Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Institute of Molecular and Cellular Bioresources, The University of Tokyo, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):839-843. doi: 10.1099/ijs.0.023564-0. Epub 2010 May 21.
A novel actinomycete that was capable of degrading poly(l-lactic acid), strain CMU-PLA07(T), was isolated from soil in northern Thailand. Strain CMU-PLA07(T) had biochemical, chemotaxonomic, morphological and physiological properties that were consistent with its classification in the genus Amycolatopsis. 16S rRNA gene sequence analysis showed that the isolate formed a phyletic line within the genus Amycolatopsis. Strain CMU-PLA07(T) was most similar to Amycolatopsis coloradensis IMSNU 22096(T) (99.5 % 16S rRNA gene sequence similarity) and Amycolatopsis alba DSM 44262(T) (99.4 %). However, strain CMU-PLA07(T) was distinguishable from the type strains of species of the genus Amycolatopsis on the basis of DNA-DNA relatedness and phenotypic data. Therefore, strain CMU-PLA07(T) is considered to represent a novel species of the genus Amycolatopsis, for which the name Amycolatopsis thailandensis sp. nov. is proposed. The type strain is CMU-PLA07(T) ( = JCM 16380(T) = BCC 38279(T)).
从泰国北部的土壤中分离到一株能够降解聚 L-乳酸的新型放线菌,菌株 CMU-PLA07(T)。菌株 CMU-PLA07(T) 的生化、化学分类、形态和生理特性与其在 Amycolatopsis 属中的分类一致。16S rRNA 基因序列分析表明,该分离株在 Amycolatopsis 属内形成了一个分支。菌株 CMU-PLA07(T) 与 Amycolatopsis coloradensis IMSNU 22096(T)(99.5% 16S rRNA 基因序列相似性)和 Amycolatopsis alba DSM 44262(T)(99.4%)最为相似。然而,菌株 CMU-PLA07(T) 与 Amycolatopsis 属的模式种在 DNA-DNA 同源性和表型数据方面存在区别。因此,菌株 CMU-PLA07(T) 被认为代表了 Amycolatopsis 属的一个新种,现命名为 Amycolatopsis thailandensis sp. nov.。其模式菌株为 CMU-PLA07(T)(=JCM 16380(T)=BCC 38279(T))。