Zhou Dan, Zhang Zehan, Zhang Jianxing, Lai Heqing, Zhou Qing, Pei Chao
Department of Stomatology, Zhejiang Hospital, Hangzhou, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Int J Behav Med. 2025 Jan 27. doi: 10.1007/s12529-025-10350-5.
Dental caries (DC) is a significant common disease of the oral cavity. Recently, researchers have focused more on the impact of poor sleep habits on the incidence and development of DC, which aroused our interest in the study of the correlation and causal relationship between sleep and dental caries.
In this study, Linkage disequilibrium score (LDSC) regression method was used to found the genetic correlation between different sleep traits and DC, while bidirectional Mendelian randomization (MR) methods were used to explore the causal relationship. The main analysis of MR was inverse variance weighted method, and the outcomes were evaluated by odds ratio (OR) and 95% confidence interval (95% CI).
In LDSC regression analysis, genetic correlations were found between chronotype, dozing, insomnia, sleep duration and DC (P = 0.002, P = 0.026, P = 7.233E-09, P = 0.012). However, when utilizing the TSMR method, no discernible casual relationships were found between chronotype, dozing, insomnia, sleep duration and DC (P = 0.832, P = 0.129, P = 0.822, P = 0.644). This result was further substantiated through the utilization of additional validation datasets.
Our study found no causal relationship between sleep traits and DC.
龋齿（DC）是口腔的一种常见重大疾病。近来，研究人员更多地关注了不良睡眠习惯对龋齿发病率和发展的影响，这引发了我们对睡眠与龋齿之间相关性及因果关系研究的兴趣。
在本研究中，使用连锁不平衡评分（LDSC）回归方法来发现不同睡眠特征与龋齿之间的遗传相关性，同时使用双向孟德尔随机化（MR）方法来探究因果关系。MR的主要分析方法是逆方差加权法，结果通过比值比（OR）和95%置信区间（95%CI）进行评估。
在LDSC回归分析中，发现昼夜节律类型、打瞌睡、失眠、睡眠时间与龋齿之间存在遗传相关性（P = 0.002，P = 0.026，P = 7.233E - 09，P = 0.012）。然而，当使用两样本孟德尔随机化（TSMR）方法时，未发现昼夜节律类型、打瞌睡、失眠、睡眠时间与龋齿之间存在明显的因果关系（P = 0.832，P = 0.129，P = 0.822，P = 0.644）。通过使用额外的验证数据集，这一结果得到了进一步证实。
我们的研究未发现睡眠特征与龋齿之间存在因果关系。
