Yin Tingting, Li Qiaowen, Sun Huan, Zheng Jin, Wang Yuanyuan, Luo Yi, Wang Li
Department of Oral & Maxillofacial Surgery, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, 430079, China.
Department of Dentistry and Endodontics , State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, 430079, China.
BMC Chem. 2025 Jan 27;19(1):25. doi: 10.1186/s13065-025-01382-9.
This work intended to assess the disinfection efficacy of hypochlorous acid (HA) and silver nanoparticles (AgNP) disinfectants in disinfecting the dental unit waterlines (DUWL) during comprehensive oral treatment and explore their potential applications in the oral medical environment.
Firstly, AgNP solution was prepared and evaluated through X-ray diffraction (XRD), field emission transmission electron microscope (FE-TEM), and stability tests. Subsequently, 15 dental units were selected and randomly assigned to three groups, each receiving a different disinfection method. Specifically, one group (5 units) received HA disinfectant (HA group), one group (5 units) received AgNP disinfectant (AgNP group), and another group (5 units) received a combination of HA and AgNP disinfectant (HA + AgNP group). Bacterial counts before and after disinfection were compared and analyzed at four sites on the dental units: high-speed handpiece tubing, mouthwash, ultrasonic scaler, and three-way syringe.
The growth of biofilm on the waterlines was observed using scanning electron microscopy (SEM) and laser confocal microscopy (LCM). The results indicated that AgNP solution was successfully prepared and demonstrated excellent stability. There was no significant difference in the average weekly number of patients treated across the three groups (P > 0.05). After disinfection, bacterial counts were significantly reduced in all groups. Compared to the HA and AgNP groups, the HA + AgNP group exhibited a markedly lower bacterial count, with statistical significance (P < 0.05). The compliance rates observed during the first disinfection and two weeks post-disinfection were slightly lower in the HA and AgNP groups compared to the HA + AgNP group, although no significant statistical difference was found (P > 0.05). SEM images revealed uneven biofilm plaques on the inner surface of the pipes prior to disinfection, embedded within a dense matrix, while the biofilm was visibly disrupted post-disinfection. LCM software analysis showed that, compared to the HA and AgNP groups, the HA + AgNP group had a significantly lower percentage of live bacteria on the biofilm post-disinfection (P < 0.05).
Compared to any single disinfectant regimen, the combined use of HA and AgNPs effectively inhibited bacterial growth and exerted a significant destructive effect on biofilms. Therefore, this combination is expected to be a viable option for disinfection of DUWL in the oral healthcare setting.
Not applicable.
本研究旨在评估次氯酸（HA）和银纳米颗粒（AgNP）消毒剂在综合口腔治疗期间对牙科单位水线（DUWL）的消毒效果，并探索它们在口腔医疗环境中的潜在应用。
首先，制备AgNP溶液并通过X射线衍射（XRD）、场发射透射电子显微镜（FE-TEM）和稳定性测试进行评估。随后，选择15个牙科单位并随机分为三组，每组接受不同的消毒方法。具体而言，一组（5个单位）接受HA消毒剂（HA组），一组（5个单位）接受AgNP消毒剂（AgNP组），另一组（5个单位）接受HA和AgNP消毒剂的组合（HA+AgNP组）。比较并分析牙科单位四个部位（高速手机管道、漱口水、超声洁牙器和三通注射器）消毒前后的细菌计数。
使用扫描电子显微镜（SEM）和激光共聚焦显微镜（LCM）观察水线上生物膜的生长情况。结果表明，成功制备了AgNP溶液并显示出优异的稳定性。三组每周平均治疗患者数量无显著差异（P>0.05）。消毒后，所有组的细菌计数均显著降低。与HA组和AgNP组相比，HA+AgNP组的细菌计数明显更低，具有统计学意义（P<0.05）。HA组和AgNP组在首次消毒和消毒后两周的依从率略低于HA+AgNP组，尽管未发现显著统计学差异（P>0.05）。SEM图像显示，消毒前管道内表面有不均匀的生物膜斑块，嵌入致密基质中，而消毒后生物膜明显被破坏。LCM软件分析表明，与HA组和AgNP组相比，HA+AgNP组消毒后生物膜上活细菌的百分比显著更低（P<0.05）。
与任何单一消毒方案相比，HA和AgNP联合使用可有效抑制细菌生长，并对生物膜产生显著的破坏作用。因此，这种组合有望成为口腔医疗环境中DUWL消毒的可行选择。
不适用。
