Charmine Pricilla, Venkatesan Vettriselvi, Geminiganesan Sangeetha, Nammalwar Bollam Rangaswamy, Dandapani Mohanapriya Chinambedu
Faculty of Clinical Research, Sri Ramachandra Institute of Higher Education and Research (SRIHER), Porur, Chennai, India.
Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research (SRIHER), Porur, Chennai, India.
Indian J Nephrol. 2025 Jan-Feb;35(1):59-63. doi: 10.25259/ijn_47_23. Epub 2024 Jun 17.
Nephrotic syndrome is a common cause of kidney diseases in children. Many studies have examined the association of microRNAs playing potential roles in many pathophysiological functions. We investigated the expression pattern of the microRNAs miR-17-5P, miR-155p, miR-424-5p in children with steroid sensitive nephrotic syndrome (SSNS) and steroid resistance nephrotic syndrome (SRNS), along with the healthy subjects.
Total RNA was isolated from the urine samples from the three groups (SSNS n = 100, SRNS n = 100, and healthy control group n = 100). Bioinformatics tools such as miRWalk and miR-Tar link were used in predicting targets for the microRNAs. Online database and g profiler software are used to evaluate the targets based on the biological functions. The expression pattern for the candidate microRNAs was carried out using quantitative real time polymerase chain reaction (RT-PCR) equipment.
miR-424 and miR-155 were upregulated in SRNS group while miR-17 was downregulated in SRNS group. miR-424-5p and miR-155p was up regulated in SRNS group while miR-17-5p was downregulated.
Combined analysis of gene expression along with studied candidate microRNAs can give better understanding of the pathogenesis of childhood nephrotic syndrome.
肾病综合征是儿童肾脏疾病的常见病因。许多研究探讨了在多种病理生理功能中发挥潜在作用的微小RNA之间的关联。我们研究了微小RNA miR-17-5P、miR-155p、miR-424-5p在激素敏感型肾病综合征（SSNS）和激素抵抗型肾病综合征（SRNS）患儿以及健康受试者中的表达模式。
从三组（SSNS组n = 100、SRNS组n = 100和健康对照组n = 100）的尿液样本中分离总RNA。使用miRWalk和miR-Tar link等生物信息学工具预测微小RNA的靶标。利用在线数据库和g profiler软件基于生物学功能评估靶标。使用定量实时聚合酶链反应（RT-PCR）设备检测候选微小RNA的表达模式。
SRNS组中miR-424和miR-155上调，而miR-17下调。SRNS组中miR-424-5p和miR-155p上调，而miR-17-5p下调。
对基因表达以及所研究的候选微小RNA进行联合分析，有助于更好地理解儿童肾病综合征的发病机制。
