Sui Weiguo, Lin Hua, Li Haiyan, Yan Qiang, Chen Jiejing, Dai Yong
Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, China.
Iran J Kidney Dis. 2014 Sep;8(5):371-6.
Recent studies have shown that serum microRNAs have specific expression patterns in some diseases, indicating the potential of using microRNAs to aid diagnosis. This study estimated the levels of microRNAs in patients with nephrotic syndrome compared with healthy controls.
In this study, real-time quantitative polymerase chain reaction was used to explore whether there were different expression levels of miR-181a, miR-483-5p, and miR-557 in the serum of patients with nephrotic syndrome subtypes and healthy controls. We measured the three microRNAs in 40 patients with nephrotic syndrome and 16 healthy controls.
The circulating levels of miR-483-5p and miR-557 were not significantly upregulated or downregulated, whereas miR-181a was significantly upregulated in patients with nephrotic syndrome as compared with healthy controls.
We found that circulating miR-181a had a significantly different expression and could be an effective means to aid diagnosis of nephrotic syndrome. This microRNA is an attractive candidate as a biomarker for nephrotic syndrome.
最近的研究表明，血清微小RNA在某些疾病中具有特定的表达模式，这表明利用微小RNA辅助诊断具有潜力。本研究评估了肾病综合征患者与健康对照者体内微小RNA的水平。
在本研究中，采用实时定量聚合酶链反应来探究肾病综合征各亚型患者及健康对照者血清中miR-181a、miR-483-5p和miR-557的表达水平是否存在差异。我们检测了40例肾病综合征患者和16例健康对照者体内的这三种微小RNA。
与健康对照者相比，肾病综合征患者血清中miR-483-5p和miR-557的循环水平未出现显著上调或下调，而miR-181a显著上调。
我们发现循环miR-181a的表达存在显著差异，可能是辅助诊断肾病综合征的有效手段。这种微小RNA作为肾病综合征的生物标志物很有吸引力。
