• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从人孤雌生殖干细胞生成囊胚样细胞。

Generation of blastoids from human parthenogenetic stem cells.

作者信息

Zhong Ke, Luo Yu-Xin, Li Dan, Min Zhe-Ying, Fan Yong, Yu Yang

机构信息

Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China.

Department of Obstetrics and Gynecology, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China.

出版信息

Life Med. 2023 Feb 18;2(1):lnad006. doi: 10.1093/lifemedi/lnad006. eCollection 2023 Feb.

DOI:10.1093/lifemedi/lnad006
PMID:39872951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11748981/
Abstract

Parthenogenetic embryos derive their genomes entirely from the maternal genome and lack paternal imprint patterns. Many achievements have been made in the study of genomic imprinting using human parthenogenetic embryonic stem cells (hPg-ESCs). However, due to developmental defects and ethical limits, a comprehensive understanding of parthenogenetic embryonic development is still lacking. Here, we generated parthenogenetic blastoids (hPg-EPSCs blastoids) from hPg-ESC-derived extended pluripotent stem cells (hPg-EPSCs) using our previously published two-step induction protocol. Morphology, specific marker expression and single-cell transcriptome analysis showed that hPg-EPSCs blastoids contain crucial cell lineages similar to blastoids (hBp-EPSCs blastoids) generated from human biparental EPSCs (hBp-EPSCs). Single-cell RNA-seq compared the expression of genes related to imprinting and X chromosome inactivation in hPg-EPSCs blastoids and hBp-EPSCs blastoids. In conclusion, we generated parthenogenetic blastoids, which will potentially promote the study of genomic imprinting in embryonic development and uncover the influence of parental origin bias on human development and pathological mechanisms.

摘要

孤雌生殖胚胎的基因组完全来自母本基因组，缺乏父本印记模式。利用人类孤雌生殖胚胎干细胞（hPg-ESCs）在基因组印记研究方面已取得许多成果。然而，由于发育缺陷和伦理限制，对孤雌生殖胚胎发育仍缺乏全面了解。在此，我们使用我们之前发表的两步诱导方案，从hPg-ESC衍生的扩展多能干细胞（hPg-EPSCs）中生成了孤雌生殖囊胚样结构（hPg-EPSCs囊胚样结构）。形态学、特异性标志物表达和单细胞转录组分析表明，hPg-EPSCs囊胚样结构包含与人类双亲EPSCs（hBp-EPSCs）产生的囊胚样结构（hBp-EPSCs囊胚样结构）相似的关键细胞谱系。单细胞RNA测序比较了hPg-EPSCs囊胚样结构和hBp-EPSCs囊胚样结构中与印记和X染色体失活相关基因的表达。总之，我们生成了孤雌生殖囊胚样结构，这可能会促进胚胎发育中基因组印记的研究，并揭示亲本来源偏差对人类发育和病理机制的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/843b83661ed7/lnad006_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/0a1159a6d488/lnad006_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/ec00ad381f9b/lnad006_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/a38130fb777a/lnad006_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/843b83661ed7/lnad006_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/0a1159a6d488/lnad006_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/ec00ad381f9b/lnad006_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/a38130fb777a/lnad006_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4656/11748981/843b83661ed7/lnad006_fig4.jpg

相似文献

1
Generation of blastoids from human parthenogenetic stem cells.从人孤雌生殖干细胞生成囊胚样细胞。
Life Med. 2023 Feb 18;2(1):lnad006. doi: 10.1093/lifemedi/lnad006. eCollection 2023 Feb.
2
Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells.表皮生长因子诱导扩展多能干细胞重构出的类胚胎体中滋养外胚层谱系转录组类似于人类胚胎。
Cell Prolif. 2022 Nov;55(11):e13317. doi: 10.1111/cpr.13317. Epub 2022 Jul 26.
3
Generation of Mouse Parthenogenetic Epiblast Stem Cells and Their Imprinting Patterns.生成鼠孤雌生殖胚胎干细胞及其印迹模式。
Int J Mol Sci. 2019 Oct 31;20(21):5428. doi: 10.3390/ijms20215428.
4
Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs.蛋白质表达图谱定义了胚胎干细胞来源的小鼠类囊胚的形成潜能。
Front Cell Dev Biol. 2022 Feb 8;10:840492. doi: 10.3389/fcell.2022.840492. eCollection 2022.
5
Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states.等位基因特异性 RNA-seq 表达谱分析在小鼠同源多能状态下的印记基因。
Epigenetics Chromatin. 2019 Feb 15;12(1):14. doi: 10.1186/s13072-019-0259-8.
6
Distinct Imprinting Signatures and Biased Differentiation of Human Androgenetic and Parthenogenetic Embryonic Stem Cells.人类雄核和孤雌胚胎干细胞的独特印记特征和偏向性分化。
Cell Stem Cell. 2019 Sep 5;25(3):419-432.e9. doi: 10.1016/j.stem.2019.06.013.
7
Conversion of genomic imprinting by reprogramming and redifferentiation.基因组印迹的重编程和再分化转化。
J Cell Sci. 2013 Jun 1;126(Pt 11):2516-24. doi: 10.1242/jcs.122754. Epub 2013 Mar 22.
8
Mouse Parthenogenetic Embryonic Stem Cells with Biparental-Like Expression of Imprinted Genes Generate Cortical-Like Neurons That Integrate into the Injured Adult Cerebral Cortex.具有双亲样印迹基因表达的小鼠孤雌生殖胚胎干细胞产生皮质样神经元，并整合到损伤的成年大脑皮层中。
Stem Cells. 2018 Feb;36(2):192-205. doi: 10.1002/stem.2721. Epub 2017 Nov 10.
9
Novel imprinted single CpG sites found by global DNA methylation analysis in human parthenogenetic induced pluripotent stem cells.通过全基因组 DNA 甲基化分析在人类孤雌诱导多能干细胞中发现的新型印迹单 CpG 位点。
Epigenetics. 2018;13(4):343-351. doi: 10.1080/15592294.2018.1460033. Epub 2018 May 3.
10
Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.使用三维两步诱导系统生成人类 EPS-Blastoids 的方案。
Methods Mol Biol. 2024;2767:27-41. doi: 10.1007/7651_2022_471.

本文引用的文献

1
Human blastoids model blastocyst development and implantation.人类桑椹胚模型模拟囊胚发育和着床。
Nature. 2022 Jan;601(7894):600-605. doi: 10.1038/s41586-021-04267-8. Epub 2021 Dec 2.
2
X-chromosome inactivation: implications in human disease.X 染色体失活：在人类疾病中的意义。
J Genet. 2021;100.
3
Reconstructing aspects of human embryogenesis with pluripotent stem cells.利用多能干细胞重建人类胚胎发生的各个方面。
Nat Commun. 2021 Sep 21;12(1):5550. doi: 10.1038/s41467-021-25853-4.
4
Generation of human blastocyst-like structures from pluripotent stem cells.从多能干细胞生成人类囊胚样结构。
Cell Discov. 2021 Sep 7;7(1):81. doi: 10.1038/s41421-021-00316-8.
5
Naive stem cell blastocyst model captures human embryo lineage segregation.原始干细胞囊胚模型捕捉人类胚胎谱系分离。
Cell Stem Cell. 2021 Jun 3;28(6):1016-1022.e4. doi: 10.1016/j.stem.2021.04.031. Epub 2021 May 5.
6
Capturing human trophoblast development with naive pluripotent stem cells in vitro.在体外利用原始多能干细胞捕获人类滋养层细胞的发育。
Cell Stem Cell. 2021 Jun 3;28(6):1023-1039.e13. doi: 10.1016/j.stem.2021.03.013. Epub 2021 Apr 7.
7
Modelling human blastocysts by reprogramming fibroblasts into iBlastoids.通过将成纤维细胞重编程为 iBlastoids 来模拟人类胚胎囊胚。
Nature. 2021 Mar;591(7851):627-632. doi: 10.1038/s41586-021-03372-y. Epub 2021 Mar 17.
8
Blastocyst-like structures generated from human pluripotent stem cells.由人类多能干细胞生成的类囊胚结构。
Nature. 2021 Mar;591(7851):620-626. doi: 10.1038/s41586-021-03356-y. Epub 2021 Mar 17.
9
X chromosome inactivation in human development.人类发育中的 X 染色体失活。
Development. 2020 Jan 3;147(1):dev183095. doi: 10.1242/dev.183095.
10
Distinct Imprinting Signatures and Biased Differentiation of Human Androgenetic and Parthenogenetic Embryonic Stem Cells.人类雄核和孤雌胚胎干细胞的独特印记特征和偏向性分化。
Cell Stem Cell. 2019 Sep 5;25(3):419-432.e9. doi: 10.1016/j.stem.2019.06.013.