使用血栓弹力图对内皮糖萼启发表面上的血液凝固进行体外评估。
Ex vivo evaluation of blood coagulation on endothelial glycocalyx-inspired surfaces using thromboelastography.
作者信息
Zang Yanyi, Vlcek Jessi R, Cuchiaro Jamie, Popat Ketul C, Olver Christine S, Kipper Matt J, Reynolds Melissa M
机构信息
School of Biomedical Engineering, Colorado State University, Fort Collins, CO USA.
Present Address: Autonomous Reanimation and Evacuation Research Program, The Geneva Foundation, Brook City-Base, San Antonio, TX USA.
出版信息
In Vitro Model. 2021 Oct 29;1(1):59-71. doi: 10.1007/s44164-021-00001-w. eCollection 2022 Feb.
PURPOSE
Present blood-contacting materials have not yet demonstrated to be effective in reducing blood coagulation without causing additional side effects clinically. We have developed an endothelial glycocalyx-inspired biomimetic surface that combines nanotopography, heparin presentation, and nitric oxide (NO)-releasing features. The resulting modified surfaces have already shown promise in reducing unfavorable blood-material interactions using platelet-rich plasma. In this study, the efficacy of modified surfaces for reducing coagulation of human whole blood was measured. In addition, the effects of leached polysaccharides and chemical modification of the modified surfaces were evaluated.
METHODS
Leached polysaccharides in the incubation solution were detected by a refractive index method to determine the potential influences of these modified surfaces on the blood coagulation observation. Chemical modifications by the nitrosation process on the polysaccharides in the modified surfaces were detected using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). Clot formation parameters were measured using thromboelastography (TEG), a clinically relevant technique to evaluate whole blood coagulation.
RESULTS
No polysaccharides were detected in the heparinized polyelectrolyte multilayer-coated titania nanotube array surface (TiONT + PEM) incubation solution; however, polysaccharides were detected from NO-releasing TiONT + PEM surface (TiONT + PEM + NO) incubation solution both after the nitrosation process and after all NO was released. The structures of thiolated chitosan and heparin were altered by -butyl nitrite. All heparin-containing surfaces were shown to slow or inhibit clot formation.
CONCLUSION
This study is the first to evaluate these endothelial glycocalyx-inspired surfaces using clinically relevant parameters, as well as proposing potential influences of these modified surfaces on the inhibition of clot formation.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s44164-021-00001-w.
目的
目前的血液接触材料在临床上尚未证明能有效减少血液凝固而不引起额外的副作用。我们开发了一种受内皮糖萼启发的仿生表面，它结合了纳米拓扑结构、肝素呈现和一氧化氮（NO）释放功能。所得的改性表面已显示出在使用富血小板血浆减少不良血液-材料相互作用方面的前景。在本研究中，测量了改性表面减少人全血凝固的功效。此外，还评估了浸出多糖的影响以及改性表面的化学修饰。
方法
采用折射率法检测孵育溶液中的浸出多糖，以确定这些改性表面对血液凝固观察的潜在影响。使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）检测改性表面中多糖的亚硝化过程化学修饰。使用血栓弹力图（TEG）测量凝血形成参数，TEG是一种评估全血凝固的临床相关技术。
结果
在肝素化聚电解质多层涂层二氧化钛纳米管阵列表面（TiONT + PEM）孵育溶液中未检测到多糖；然而，在亚硝化过程后以及所有NO释放后，从释放NO的TiONT + PEM表面（TiONT + PEM + NO）孵育溶液中检测到多糖。硫醇化壳聚糖和肝素的结构被亚硝酸丁酯改变。所有含肝素的表面均显示出减缓或抑制凝血形成。
结论
本研究首次使用临床相关参数评估这些受内皮糖萼启发的表面，并提出这些改性表面对抑制凝血形成的潜在影响。
补充信息
在线版本包含可在10.1007/s44164-021-00001-w获取的补充材料。
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