Sumner A T
Chromosoma. 1985;91(2):145-50. doi: 10.1007/BF00294059.
The distribution of quinacrine and protein sulphur has been compared with that of DNA in euchromatic and heterochromatic regions of mouse chromosomes stained with the fluorescent dye quinacrine, using X-ray microanalysis. Heterochromatin tends to bind relatively more quinacrine than euchromatin, and contains a greater concentration of sulphur. Measurements of quinacrine fluorescence, when compared with quinacrine binding, show that the excitation of fluorescence is more efficient when the dye is bound to euchromatin than when it is bound to heterochromatin. Although this observation is consistent with the hypothesis that the dull quinacrine fluorescence of mouse centromeres is due to quenching by guanine residues, two other factors should also be considered: the lower absolute amount of dye bound to the centromeres, and a concentration-dependent quenching of fluorescence.
利用X射线微量分析，将喹吖因和蛋白质硫的分布与用荧光染料喹吖因染色的小鼠染色体常染色质和异染色质区域中DNA的分布进行了比较。异染色质比常染色质倾向于结合相对更多的喹吖因，并且含有更高浓度的硫。与喹吖因结合相比，喹吖因荧光测量结果表明，当染料与常染色质结合时，荧光激发比与异染色质结合时更有效。尽管这一观察结果与小鼠着丝粒暗淡的喹吖因荧光是由于鸟嘌呤残基淬灭这一假设一致，但还应考虑另外两个因素：着丝粒结合的染料绝对量较低，以及荧光的浓度依赖性淬灭。
