Ferrucci L, Mezzanotte R
J Histochem Cytochem. 1982 Dec;30(12):1289-92. doi: 10.1177/30.12.6818276.
Photooxidation is believed to preferentially remove guanine (G) residues from chromosomal DNA. G interspersion, moreover, has been hypothesized as quenching quinacrine (Q) fluorescence in cytological preparations. Hence, we used photooxidation as a tool for inducing possible changes in the Q-banding pattern of Drosophila melanogaster, Drosophila virilis, and Mus musculus metaphase chromosomes. An enhanced Q fluorescence, which was particularly evident in certain chromosomal regions, was found. This finding would support the postulated primary role of G in determining Q bands in eukaryotic chromosomes.
据信，光氧化作用会优先从染色体DNA中去除鸟嘌呤（G）残基。此外，有人推测G的散布会淬灭细胞学标本中的喹吖因（Q）荧光。因此，我们将光氧化作为一种工具，用于诱导黑腹果蝇、粗壮果蝇和小家鼠中期染色体的Q带模式发生可能的变化。我们发现Q荧光增强，在某些染色体区域尤为明显。这一发现将支持关于G在真核染色体Q带确定中假定的主要作用的观点。
