一种关于鸟嘌呤在真核染色体中决定喹吖因荧光反应作用的细胞学研究方法。

A cytological approach to the role of guanine in determining quinacrine fluorescence response in eukaryotic chromosomes.

作者信息

Ferrucci L, Mezzanotte R

出版信息

J Histochem Cytochem. 1982 Dec;30(12):1289-92. doi: 10.1177/30.12.6818276.

DOI:10.1177/30.12.6818276

Abstract

Photooxidation is believed to preferentially remove guanine (G) residues from chromosomal DNA. G interspersion, moreover, has been hypothesized as quenching quinacrine (Q) fluorescence in cytological preparations. Hence, we used photooxidation as a tool for inducing possible changes in the Q-banding pattern of Drosophila melanogaster, Drosophila virilis, and Mus musculus metaphase chromosomes. An enhanced Q fluorescence, which was particularly evident in certain chromosomal regions, was found. This finding would support the postulated primary role of G in determining Q bands in eukaryotic chromosomes.

摘要

据信，光氧化作用会优先从染色体DNA中去除鸟嘌呤（G）残基。此外，有人推测G的散布会淬灭细胞学标本中的喹吖因（Q）荧光。因此，我们将光氧化作为一种工具，用于诱导黑腹果蝇、粗壮果蝇和小家鼠中期染色体的Q带模式发生可能的变化。我们发现Q荧光增强，在某些染色体区域尤为明显。这一发现将支持关于G在真核染色体Q带确定中假定的主要作用的观点。